Tetrazine-Ph-PEG5-NHS ester
Based on 1 Customer Validation
Tetrazine-Ph-PEG5-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. Tetrazine-Ph-PEG5-NHS ester is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.
For research use only. We do not sell to patients.
- Purity : 99.29%
- CAS No.: 1682653-80-0
- Formula: C27H36N6O10
- Molecular Weight:604.61
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-141269
Methyltetrazine-PEG5-NHS ester -
HY-130283
Methyltetrazine-Ph-NHS esterPROTAC Linker -
HY-141264
Methyltetrazine-PEG4-acid -
HY-141268
Methyltetrazine-Sulfo-NHS ester sodium -
HY-140214
Azido-PEG5-aminePROTAC Linkers -
HY-126908
Tetrazine-Ph-NHS esterPROTAC Linker -
HY-141263
Methyltetrazine-acid -
HY-141270
Methyltetrazine-PEG8-NHS esterPROTAC Linkers -
HY-W072752
endo-BCN-PEG2-NH2 -
HY-W110146
(3aS,4S,6aR)-Biotin-PEG4-AlkyneClick Chemistry Compound -
HY-W111141
BCN-OHBCN-TPP Probe Control -
HY-124480
Tetrazine-Ph-acidPROTAC Linker
All PROTAC Linkers Isoforms
More
Biological Activity
Description
IC50 & Target
|
PEGs |
Alkyl/ether |
In Vitro
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 1682653-80-0
-
Appearance Solid
-
Molecular Weight 604.61
-
Formula C27H36N6O10
-
Color Purple to purplish red
-
SMILES
O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCOCCC(NCC2=CC=C(C3=NN=CN=N3)C=C2)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
-
Data Sheet (263 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Tetrazine-Ph-PEG5-NHS ester Related Classifications
HY-130551 Related Classifications
Powered by Bioz