TMR Biocytin
TMR Biocytin is a polar tracer used in the research of cell-cell and cell-liposome fusions, as well as membrane permeability and cellular uptake during pinocytosis. TMR Biocytin can be detected using streptavidin, and is an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm).
For research use only. We do not sell to patients.
- CAS No.: 749247-49-2
- Formula: C46H60N8O7S
- Molecular Weight:869.08
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Dilute 1 mg TMR Biocytin in 100 μL PBS (per mouse), and inject the solution into the tail vein.
2. 30 min after injection, anesthetize and perfuse the animals.
3. Remove spinal cords, prepare deep frozen and serial 10 μm longitudinal sections.
4. Stain nuclear counterstain using DAPI.
5. Obtaine the images of whole sections with ×10 power of objective, ×10 power of eyepiece, by using identical laser intensity, exposure times and magnification in all cohorts.
6. To set the above parameters, livers from tracer injected mice and non-injected mice were used.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
571
544
Chemical Information
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CAS No. 749247-49-2
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Molecular Weight 869.08
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Formula C46H60N8O7S
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SMILES
N[C@@H](CCCCNC(CCCC[C@]1([H])SC[C@]([C@]1([H])N2)([H])NC2=O)=O)C(NCCCCCNC(C3=CC(C([O-])=O)=C(C4=C(C=C/5)C(OC6=C4C=CC(N(C)C)=C6)=CC5=[N+](C)/C)C=C3)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Mads Harsløf, et al. Fast neuronal labeling in live tissue using a biocytin conjugated fluorescent probe. J Neurosci Methods. 2015 Sep 30;253:101-9. [Content Brief]
[2]. Smadar Goldfarb, et al. Electric neurostimulation regulates microglial activation via retinoic acid receptor α signaling. Brain Behav Immun. 2021 Aug;96:40-53. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TMR Biocytin Related Classifications
HY-D1672 Related Classifications
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