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TMR Biocytin is a polar tracer used in the research of cell-cell and cell-liposome fusions, as well as membrane permeability and cellular uptake during pinocytosis. TMR Biocytin can be detected using streptavidin, and is an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm).

For research use only. We do not sell to patients.

TMR Biocytin Chemical Structure

TMR Biocytin Chemical Structure

CAS No. : 749247-49-2

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Description

TMR Biocytin is a polar tracer used in the research of cell-cell and cell-liposome fusions, as well as membrane permeability and cellular uptake during pinocytosis. TMR Biocytin can be detected using streptavidin, and is an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm)[1].

In Vivo

TMR Biocytin can be used to examine the permeability changes of blood brain barrier (BBB)[2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Dilute 1 mg TMR Biocytin in 100 μL PBS (per mouse), and inject the solution into the tail vein.
2. 30 min after injection, anesthetize and perfuse the animals.
3. Remove spinal cords, prepare deep frozen and serial 10 μm longitudinal sections.
4. Stain nuclear counterstain using DAPI.
5. Obtaine the images of whole sections with ×10 power of objective, ×10 power of eyepiece, by using identical laser intensity, exposure times and magnification in all cohorts.
6. To set the above parameters, livers from tracer injected mice and non-injected mice were used.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

869.08

Formula

C46H60N8O7S

CAS No.
Emission (Em)

571

Excitation (Ex)

544

SMILES

N[C@@H](CCCCNC(CCCC[C@]1([H])SC[C@]([C@]1([H])N2)([H])NC2=O)=O)C(NCCCCCNC(C3=CC(C([O-])=O)=C(C4=C(C=C/5)C(OC6=C4C=CC(N(C)C)=C6)=CC5=[N+](C)/C)C=C3)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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TMR Biocytin Related Classifications

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This equation is commonly abbreviated as: C1V1 = C2V2

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
TMR Biocytin
Cat. No.:
HY-D1672
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