TPA-IM
TPA-IM is an Aβ40-binding inhibitor with a Ka of 12.7 nM. TPA-IM inhibits amyloid-β fibrillation and disrupts preformed fibrils. TPA-IM inhibits intracellular ROS production, restores mitochondrial membrane potential, attenuates amyloid-β-induced apoptosis (apoptosis), and rescues amyloid-β-induced loss of neuronal viability. TPA-IM self-assembles into spherical nanoaggregates and co-assembles with amyloid-β, redirecting aggregation toward non-fibrillar structures. TPA-IM can be used for research on Alzheimer's disease.
For research use only. We do not sell to patients.
- Formula: C33H23N3O3
- Molecular Weight:509.55
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
TPA-IM (30 μM) self-assembles into spherical nanoaggregates of approximately 100-150 nm and co-assembles with Aβ40 into non-fibrillar hybrid structures[1].
TPA-IM (0-100 μM; 48 h) shows no cytotoxicity in SH-SY5Y and IMR-32 cells up to 100 μM and rescues Aβ40-induced loss of viability in SH-SY5Y cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y and IMR-32 human neuroblastoma cells
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Concentration:0, 10, 20, 30, 40, 50, 75, 100 μM (TPA-IM); 10-100 μM (MTT interference study)
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Incubation Time:48 h (TPA-IM); 2 h (MTT); 15 min (DMSO)
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Result:Exhibited negligible intrinsic cytotoxicity in SH-SY5Y cells over 1-100 μM, maintaining high cellular viability even at elevated doses.
In IMR-32 cells, TPA-IM maintained comparatively higher cell viability.
Aβ40 fibrils dose-dependently reduced neuronal viability; co-incubation with TPA-IM significantly rescued cell viability relative to the Aβ40 group.
- Mitochondrial membrane-potential fluorescent assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Fluorescent plasma-membrane potential dye assay
- Mitochondrial membrane-potential and mitochondrial mass staining
- Apoptosis Solutions
- Amyloid: Congo Red Amyloid Staining
- Alzheimer’s Disease Modeling
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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Molecular Weight 509.55
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Formula C33H23N3O3
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SMILES
O=C(C1=C(C/2=C(C#N)/C#N)C=CC=C1)C2=C/C3=CC=C(N(C4=CC=C(OC)C=C4)C5=CC=C(OC)C=C5)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TPA-IM Related Classifications
HY-189811 Related Classifications
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