1. Dye Reagents
  2. TRITC-DHPE

TRITC-DHPE 

Cat. No.: HY-D1671
Handling Instructions

TRITC-DHPE is a rhodamine-labeled glycerophosphate ethanolamine lipid, with head groups marked with bright red fluorescent TRITC dye (λEx/λEm=514/580 nm). TRITC-DHPE can be used for membrane fusion assay to trace lipid processing in intracellular phagocytosis. TRITC-DHPE can serves as an energy transfer receptor for NBD, BODIPY and fluorescein lipid probes.

For research use only. We do not sell to patients.

TRITC-DHPE Chemical Structure

TRITC-DHPE Chemical Structure

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

TRITC-DHPE is a rhodamine-labeled glycerophosphate ethanolamine lipid, with head groups marked with bright red fluorescent TRITC dye (λEx/λEm=514/580 nm). TRITC-DHPE can be used for membrane fusion assay to trace lipid processing in intracellular phagocytosis. TRITC-DHPE can serves as an energy transfer receptor for NBD, BODIPY and fluorescein lipid probes[1][2].

In Vitro

TRITC-DHPE can be served as a unilamellar liposome, and labels cells[1].

Protocol of flow cytometry assay[1]:
1.Dissolve TRITC-DHPE probe in ethanol;
2.Sonicate and dilute the probe with electroporation buffer and sonicate the probe again, prepare the probe final stock concentration of 4.6 μM;
3.Add 15 mL probe stock solution into a T-75 flask, label cells at 37 ℃ for 2.5 h;
4.Wash cells twice with PBS, trypsinize cells, and wash cells again prior to analysis;
5.Pellet and resuspend cells in Ca2+-, Mg2+-free PBS, analyze via flow cytometry with 514 nm laser excitation and 585/42 nm filter;
6.Adjust aqueous suspension with pH range of 4-6.5;
7.Construct TRITC-DHPE pH-independent emission standard curves and set the excitation wavelength at 514 nm, set emission wavelength at 580 nm, and slit widths of 4 nm.

Protocol of TRITC-DHPE preparation[2]:
1.Store TRITC-DHPE in chloroform (1 mg/ml, stock);
2.Dry dye stock solution (1-5 μL) into a film immediately before use, and reconstitute in 20–100 μL of ethanol;
3.Incubate cells with a final concentration of 100 nM-1 μM for 5-10 min at 22 ℃ in supplemented RPMI 1640 media with FCS;
4.The maximum concentration of ethanol during incubation was 1% v/v.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1233.69

Formula

C69H111N5O10PS

SMILES

CCCCCCCCCCCCCCCC(O[[email protected]@H](COP([O-])(OCCNC(NC1=CC=C(C([O-])=O)C(C2=C(C=C/3)C(CC4=CC(N(C)C)=CC=C42)=CC3=[N+](C)/C)=C1)=S)=O)COC(CCCCCCCCCCCCCCC)=O)=O.CC[N+](CC)CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
TRITC-DHPE
Cat. No.:
HY-D1671
Quantity:
MCE Japan Authorized Agent: