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  3. XTT sodium hydrate

XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.

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CAS No. : 413585-64-5

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Top Publications Citing Use of Products

    XTT sodium hydrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jul 1;23(1):465.  [Abstract]

    Biofilm eradication rates of PMB@Td and PMB@Lipo at different concentrations was determined by XTT sodium (100 µL; 1 mg/mL) assays.

    XTT sodium hydrate purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 Apr 8.  [Abstract]

    XTT sodium (150 μL; 0.5 mg/mL; 3 h; 37 ℃) was used to assess biofilm inhibition and eradication of C. neoformans H99 by mesoricin1 and mesoricin4 at different concentrations.
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    Description

    XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.

    Formula

    C22H18N7NaO14S2

    CAS No.
    SMILES

    O=C(C1=NN([N+](C2=C(OC)C=C([N+]([O-])=O)C(S(=O)([O-])=O)=C2)=N1)C3=C(OC)C=C([N+]([O-])=O)C(S(=O)(O[Na])=O)=C3)NC4=CC=CC=C4.O.[x]

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    Room temperature in continental US; may vary elsewhere.

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    Please store the product under the recommended conditions in the Certificate of Analysis.

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    XTT sodium hydrate
    Cat. No.:
    HY-145499
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