Cat. No.: HY-115543

β-catenin-IN-37

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β-catenin-IN-37 is a selective β-Catenin/T-cell factor protein-protein interaction (β-catenin/Tcf PPI) inhibitor. β-catenin-IN-37 inhibits canonical Wnt signaling and the growth of colorectal cancer cells SW480 and HCT116 with the IC₅₀ values of 20 μM and 31 μM, respectively.

For research use only. We do not sell to patients.

Purity: 99.13%
CAS No.: 1783856-40-5
Formula: C₁₃H₉N₉O
Molecular Weight:307.27

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

β-catenin-IN-37 Chemical Structure

CAS No.: 1783856-40-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

β-catenin-IN-37 inhibits SW480 and HCT116 with the IC₅₀ values of 20 μM and 31 μM, respectively.

All Wnt Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	EC₅₀	31 μM Compound: 70	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 24 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 24 hrs by MTT assay	[PMID: 33569941]
HCT-116	IC₅₀	31 μM Compound: 37	Cytotoxicity against human HCT116 cells assessed as inhibition of cell growth after 72 hrs by MTS assay Cytotoxicity against human HCT116 cells assessed as inhibition of cell growth after 72 hrs by MTS assay	[PMID: 25985283]
HEK293	IC₅₀	26 μM Compound: 37	Inhibition of interaction of beta-catenin (unknown origin) transfected in Wnt-activated HEK293 cells/Tcf4 assessed as decrease in transactivation of canonical Wnt signaling by TOPflash luciferase reporter assay Inhibition of interaction of beta-catenin (unknown origin) transfected in Wnt-activated HEK293 cells/Tcf4 assessed as decrease in transactivation of canonical Wnt signaling by TOPflash luciferase reporter assay	[PMID: 25985283]
SW480	EC₅₀	20 μM Compound: 70	Antiproliferative activity against human SW480 cells assessed as inhibition of cell proliferation incubated for 24 hrs by MTT assay Antiproliferative activity against human SW480 cells assessed as inhibition of cell proliferation incubated for 24 hrs by MTT assay	[PMID: 33569941]
SW480	IC₅₀	>200 μM Compound: 37	Cytotoxicity against human SW480 cells assessed as inhibition of cell growth after 72 hrs by MTS assay Cytotoxicity against human SW480 cells assessed as inhibition of cell growth after 72 hrs by MTS assay	[PMID: 25985283]

In Vitro

β-catenin-IN-37 displays K_i values of 17 μM and 18 μM forβ-catenin/Tcf PPI in FP assay and in AlphaScreen assay, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1783856-40-5
Appearance Solid
Molecular Weight 307.27
Formula C₁₃H₉N₉O
Color Orange to red
SMILES

N1(CCC2=NNN=N2)C3=NC4=NON=C4N=C3C5=C1C=CC=C5
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (325.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2545 mL

16.2723 mL

32.5447 mL

5 mM

0.6509 mL

3.2545 mL

6.5089 mL

10 mM

0.3254 mL

1.6272 mL

3.2545 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Catrow JL, et al. Discovery of Selective Small-Molecule Inhibitors for the β-Catenin/T-Cell Factor Protein-Protein Interaction through the Optimization of the Acyl Hydrazone Moiety. J Med Chem. 2015;58(11):4678-4692. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2545 mL	16.2723 mL	32.5447 mL	81.3617 mL
	5 mM	0.6509 mL	3.2545 mL	6.5089 mL	16.2723 mL
	10 mM	0.3254 mL	1.6272 mL	3.2545 mL	8.1362 mL
	15 mM	0.2170 mL	1.0848 mL	2.1696 mL	5.4241 mL
	20 mM	0.1627 mL	0.8136 mL	1.6272 mL	4.0681 mL
	25 mM	0.1302 mL	0.6509 mL	1.3018 mL	3.2545 mL
	30 mM	0.1085 mL	0.5424 mL	1.0848 mL	2.7121 mL
	40 mM	0.0814 mL	0.4068 mL	0.8136 mL	2.0340 mL
	50 mM	0.0651 mL	0.3254 mL	0.6509 mL	1.6272 mL
	60 mM	0.0542 mL	0.2712 mL	0.5424 mL	1.3560 mL
	80 mM	0.0407 mL	0.2034 mL	0.4068 mL	1.0170 mL
	100 mM	0.0325 mL	0.1627 mL	0.3254 mL	0.8136 mL

Keywords

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β-catenin-IN-37

Bioactive Compound Library Max

Bioactive Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Cancer Stem Cells Compound Library

Antihypertensive Compound Library

Highly Selective Inhibitors Library

Extracellular Vesicles (EVs) Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

MCE Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

All Wnt Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

β-catenin-IN-37

Bioactive Compound Library Max

Bioactive Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Cancer Stem Cells Compound Library

Antihypertensive Compound Library

Highly Selective Inhibitors Library

Extracellular Vesicles (EVs) Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

MCE Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

All Wnt Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

β-catenin-IN-37 Related Classifications

HY-115543 Related Classifications