13-Hydroxy-alpha-tocopherol
13-Hydroxy-alpha-tocopherol is a fat-soluble vitamin E derivative and anticancer agent with antioxidant and antiproliferative activities. 13-Hydroxy-alpha-tocopherol scavenges peroxyl free radicals and protects polyunsaturated fatty acids from oxidative damage. 13-Hydroxy-alpha-tocopherol not only inhibits the growth of glioma cancer cells, but its deficiency is also closely associated with recurrent miscarriage, embryonic growth retardation and death. Clinical observations show that the expression of 13-Hydroxy-alpha-tocopherol is downregulated in the follicular fluid of patients with recurrent miscarriage. 13-Hydroxy-alpha-tocopherol has important application potential in the research of recurrent miscarriage and glioma.
For research use only. We do not sell to patients.
- CAS No.: 1160974-39-9
- Formula: C29H50O3
- Molecular Weight:446.71
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
13-Hydroxy-alpha-tocopherol (Compound 7) (10 μM; 24 h) potently inhibits the proliferation of murine glioma C6 cells, reducing cell number to ~50% of untreated controls[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:murine glioma C6 cancer cells
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Concentration:10 μM
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Incubation Time:24 h
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Result:Reduced cell proliferation to approximately 50% of the untreated control level.
Chemical Information
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CAS No. 1160974-39-9
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Molecular Weight 446.71
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Formula C29H50O3
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SMILES
CC1=C2C(O[C@@](C)(CC2)CCCC(C)CCCC(C)CCCC(C)CO)=C(C(C)=C1O)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Song J, et al. Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization. Sci Rep. 2019;9(1):10873. Published 2019 Jul 26. [Content Brief]
[2]. Mazzini F, et al. Configuration of the vitamin E analogue garcinoic acid extracted from Garcinia Kola seeds. Chirality. 2009;21(5):519-524. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
13-Hydroxy-alpha-tocopherol Related Classifications
HY-Z11709 Related Classifications
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