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  3. 14-Deoxy-11,12-didehydroandrographolide

14-Deoxy-11,12-didehydroandrographolide (Synonyms: 14-dehydro Andrographolide; AP10)

Cat. No.: HY-N1490 Purity: >98.0%
Handling Instructions

14-Deoxy-11,12-didehydroandrographolide is an analogue of Andrographolide that can be isolated from A. paniculata. 14-Deoxy-11,12-didehydroandrographolide inhibits NF-κB activation.

For research use only. We do not sell to patients.

14-Deoxy-11,12-didehydroandrographolide Chemical Structure

14-Deoxy-11,12-didehydroandrographolide Chemical Structure

CAS No. : 42895-58-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 167 In-stock
Estimated Time of Arrival: December 31
1 mg USD 72 In-stock
Estimated Time of Arrival: December 31
5 mg USD 228 In-stock
Estimated Time of Arrival: December 31
10 mg USD 336 In-stock
Estimated Time of Arrival: December 31
25 mg USD 696 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

14-Deoxy-11,12-didehydroandrographolide is an analogue of Andrographolide that can be isolated from A. paniculata. 14-Deoxy-11,12-didehydroandrographolide inhibits NF-κB activation.

IC50 & Target

NF-κB

 

In Vitro

14-deoxy-11,12-didehydroandrographolide, a naturally occurring noncytotoxic analogue of Andrographolide, effectively reduces Ovalbumin (OVA)-induced inflammatory cell recruitment into bronchoalveolar lavage (BAL) fluid, IL-4, IL-5, IL-13, and eotaxin production, serum IgE synthesis, pulmonary eosinophilia, mucus hypersecretion, mast cell degranulation, and airway hyper-responsiveness (AHR) in a mouse asthma model, probably via inhibition of NF-κB activity[1].

In Vivo

14-deoxy-11,12-didehydroandrographolide (1 mg/kg) dramatically reduces resistance (Rl) and restores Cdyn in OVA-challenged mice in response to methacholine[1].

Molecular Weight

332.43

Formula

C₂₀H₂₈O₄

CAS No.

42895-58-9

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMF : 100 mg/mL (300.82 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0082 mL 15.0408 mL 30.0815 mL
5 mM 0.6016 mL 3.0082 mL 6.0163 mL
10 mM 0.3008 mL 1.5041 mL 3.0082 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

A549 cells (3×103/well), BEAS-2B cells (5×103/well), and RBL-2H3 cells (3×103/well) are seeded in flat-bottomed 96-well plates overnight and then incubated with increasing concentrations (3-120 μM) of 14-deoxy-11,12-didehydroandrographolide or Andrographolide for 24 and 48 h at 37°C. Cell viability is analyzed using the CellTiter 96 AQueous cell proliferation assay. This MTS assay is based on the ability of viable cells to convert a soluble tetrazolium salt to a colored formazan product. Absorbance is recorded at 490 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
Female BALB/c mice, 6 to 8 weeks old, are sensitized and challenged with OVA. Briefly, mice are sensitized by ip injections of 20 μg of OVA and 4 mg of Al(OH)3 suspended in 0.1 mL of saline on days 0 and 14. On days 22, 23, and 24, mice are challenged with 1% OVA aerosol for 30 min. 14-deoxy-11,12-didehydroandrographolide (0.1, 0.5, and 1 mg/kg) or vehicle (1% DMSO) in 0.1 mL of saline is given by ip injections 2 h before and 10 h after each OVA aerosol challenge. Saline aerosol is used as a negative control.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

14-Deoxy-11,12-didehydroandrographolide14-dehydro AndrographolideAP10AP 10AP-10NF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Product name:
14-Deoxy-11,12-didehydroandrographolide
Cat. No.:
HY-N1490
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