Cat. No.: HY-N0721

Neoandrographolide

(Synonyms: Neoandrographiside)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Neoandrographolide is a diterpenoid compound isolated from Andrographis paniculata. Neoandrographolide inhibits osteoclasts differentiation and bone resorption through inhibition of MAPK/NF-κB/PI3K/AKT/GSK3β/PPAR/CAMK signaling pathway. Neoandrographolide inhibits apoptosis in rat embryonic ventricular cardiomyocytes. Neoandrographolide inhibits iNOS and the generation of ROS, activates eNOS, exhibiting anti-inflammatory and hypolipidemic activity.

For research use only. We do not sell to patients.

Purity: 99.41%
CAS No.: 27215-14-1
Formula: C₂₆H₄₀O₈
Molecular Weight:480.59

Storage:

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Neoandrographolide Chemical Structure

CAS No.: 27215-14-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All ERK Isoforms :

All p38 MAPK Isoforms :

All JNK Isoforms :

All NF-κB Isoforms :

All PI3K Isoforms :

All PPAR Isoforms :

All GSK-3 Isoforms :

All CaMK Isoforms :

All NO Synthase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	GI₅₀	26.67 μM Compound: 5	Antiproliferative activity against human HL60 cells by trypan blue assay Antiproliferative activity against human HL60 cells by trypan blue assay	[PMID: 18357994]

In Vitro

Neoandrographolide (0-30 μM, 6 days) inhibits osteoclast differentiation-related gene expression of NFATc1, c-Fos, CTSK and MMP9, inhibits RANKL-induced osteoclasts maturation in murine bone marrow macrophage^[2].
Neoandrographolide (10-90 μM, 24 h) inhibits the expression of inflammatory factors TNF-α and IL-1β^[3].
Neoandrographolide (7.5-150 μM, 24-36 h) inhibits PMA (HY-18739)-stimulated respiratory burst, inhibits LPS (HY-D1056)-induced TNF-α and NO production in cell RAW264.7^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[2]

Cell Line:

murine bone marrow macrophage
Concentration:

0-30 μM
Incubation Time:

6 days
Result:

Inhibited the expression of NFATc1, c-Fos, CTSK and MMP9.

In Vivo

Neoandrographolide (15-30 mg/kg, ip, 3 times a week for 8 weeks) alleviates OVX-induced osteoporosis in mouse models^[2].
Neoandrographolide (10 mg/kg, ip, once a day for 6 days) alleviates the ischemia-reperfusion injury in mouse models^[3].
Neoandrographolide (50-150 mg/kg, po, single dose) inhibits Dimethyl benzene-induced ear edema, exhibits anti-inflammatory activity in mouse models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

OVX-induced osteoporosis in mouse models^[2]
Dosage:

15-30 mg/kg
Administration:

ip, 3 times a week for 8 weeks
Result:

Alleviated OVX-induced bone loss.

Animal Model:

Mouse myocardial ischemia-reperfusion injury model^[3]
Dosage:

10 mg/kg
Administration:

ip, once a day for 6 days
Result:

Improved the cardiac contractile function, reduced the levels of myocardial injury markers (CK-MB and cTNI), reduced infarction area.

Animal Model:

Dimethyl benzene-induced ear edema in mouse models^[4]
Dosage:

50-150 mg/kg
Administration:

po, single dose
Result:

Inhibited ear edema at a dose of 150 mg/kg.

Chemical Information

CAS No. 27215-14-1
Appearance Solid
Molecular Weight 480.59
Formula C₂₆H₄₀O₈
Color White to off-white
SMILES

C[C@@]([C@@H]1CCC2=CCOC2=O)(CCC3)[C@@](CCC1=C)([H])[C@]3(C)CO[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO
Synonyms

Neoandrographiside
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Plants
- Acanthaceae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Publications (1)

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Journal Impact Factor

Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (208.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0808 mL

10.4039 mL

20.8078 mL

5 mM

0.4162 mL

2.0808 mL

4.1616 mL

10 mM

0.2081 mL

1.0404 mL

2.0808 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tao Yang, et al. Hypolipidemic Effects of Andrographolide and Neoandrographolide in Mice and Rats. Phytotherapy Research Volume 27, Issue 4, pages 618–623, April 2013

[2]. Tang K, et al., Neoandrographolide inhibits mature osteoclast differentiation to alleviate bone loss and treat osteoporosis. Front Pharmacol. 2025 Feb 11;16:1466057. [Content Brief]

[3]. Liu Y, et al., Amelioratory effect of neoandrographolide on myocardial ischemic-reperfusion injury by its anti-inflammatory and anti-apoptotic activities. Environ Toxicol. 2021 Dec;36(12):2367-2379. [Content Brief]

[4]. Liu J, et al., In vivo and in vitro anti-inflammatory activities of neoandrographolide. Am J Chin Med. 2007;35(2):317-28. [Content Brief]

[5]. Yang T, et al., Hypolipidemic effects of andrographolide and neoandrographolide in mice and rats. Phytother Res. 2013 Apr;27(4):618-23. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0808 mL	10.4039 mL	20.8078 mL	52.0194 mL
	5 mM	0.4162 mL	2.0808 mL	4.1616 mL	10.4039 mL
	10 mM	0.2081 mL	1.0404 mL	2.0808 mL	5.2019 mL
	15 mM	0.1387 mL	0.6936 mL	1.3872 mL	3.4680 mL
	20 mM	0.1040 mL	0.5202 mL	1.0404 mL	2.6010 mL
	25 mM	0.0832 mL	0.4162 mL	0.8323 mL	2.0808 mL
	30 mM	0.0694 mL	0.3468 mL	0.6936 mL	1.7340 mL
	40 mM	0.0520 mL	0.2601 mL	0.5202 mL	1.3005 mL
	50 mM	0.0416 mL	0.2081 mL	0.4162 mL	1.0404 mL
	60 mM	0.0347 mL	0.1734 mL	0.3468 mL	0.8670 mL
	80 mM	0.0260 mL	0.1300 mL	0.2601 mL	0.6502 mL
	100 mM	0.0208 mL	0.1040 mL	0.2081 mL	0.5202 mL

Neoandrographolide

1 Cited Publications

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Neoandrographolide

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