2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (Synonyms: Neu5Ac2en; DANA)

Name: 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid
Brand: MedChemExpress
SKU: HY-125798
Rating: 5.0 (4 reviews)

Cat. No.: HY-125798 Purity: 98.0%: Data Sheet
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N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid (Neu5Ac2en) is a potent neuraminidase (sialidase) inhibitor. N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid shows inhibitory activities against human neuraminidase enzymes with IC₅₀s of 143, 43, 61, and 74 μM for NEU1, NEU2, NEU3, and NEU4, respectively. Anti-influenza virus activity.

For research use only. We do not sell to patients.

CAS No. : 24967-27-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid

: 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Oct 9. [Abstract]; Coimmunoprecipitation assays were performed to explore the Ub levels of exogenous Flag-tagged Nectin-4 in KU-19-19 cells with or without transfection of HA-tagged Ub and the treatment of MG132 (10 μmol/L), 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (Neu5Ac2en) (80 μmol/L), or P-3Fax-Neu5Ac (200 μmol/L). The expression and Ub levels of Flag-tagged Nectin-4 were detected by Western blot. All cell samples were treated with MG132 (10 μmol/L) before collecting cells. The results showed that after the addition of Neu5Ac2en to inhibit neuraminidases and enhance sialylation in bladder cancer cells, there was an increase in the ubiquitination levels of exogenous Nectin-4.

: 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2023 Dec;14(6):2851-2865. [Abstract]; After inhibit Neu2 function by 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (DANA, 50 μM), then cultured with 15 mM lactate and differentiation for 3 days. Then detected MyHC protein level by western blot. The results showed that DANA diminished MyHC protein expression despite lactate treatment.

: 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2023 Dec;14(6):2851-2865. [Abstract]; Representative photographs of MyHC, immunofluorescence staining in C2C12 cells differentiated for 3 days under L-lactate (10 mM) and 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (DANA, 50 μM) treatment. The results showed that the growing number of myotubes induced by lactate treatment was decreased in the presence of DAN. Scale bar = 100 μm.

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	0.04 mM Compound: 2, Neu5Ac2en, DANA	Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis	[PMID: 19450982]
HEK-293T	IC₅₀	0.06 mM Compound: 2, Neu5Ac2en, DANA	Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography	[PMID: 19450982]
HEK-293T	IC₅₀	0.07 mM Compound: 2, Neu5Ac2en, DANA	Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis	[PMID: 19450982]
HEK-293T	IC₅₀	0.143 mM Compound: 2, Neu5Ac2en, DANA	Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis	[PMID: 19450982]
HEK-293T	IC₅₀	49 μM Compound: 1; DANA	Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay	[PMID: 29425031]
HEK-293T	IC₅₀	50 μM Compound: 2; DANA	Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis	[PMID: 27095056]
HEK-293T	IC₅₀	50 μM Compound: 2; DANA	Inhibition of human Neu2 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis Inhibition of human Neu2 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis	[PMID: 27095056]
HEK-293T	IC₅₀	50 μM Compound: 2; DANA	Inhibition of human Neu3 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis Inhibition of human Neu3 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis	[PMID: 27095056]
HEK-293T	IC₅₀	50 μM Compound: 2; DANA	Inhibition of human Neu4 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis Inhibition of human Neu4 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis	[PMID: 27095056]
HEK-293T	IC₅₀	50 μM Compound: 2; DANA	Inhibition of human Neu4 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis Inhibition of human Neu4 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis	[PMID: 27095056]
HEK293	IC₅₀	143 μM Compound: 1, DANA	Inhibition of human NEU1 expressed in HEK293 cells Inhibition of human NEU1 expressed in HEK293 cells	[PMID: 18068975]
HEK293	IC₅₀	168 μM Compound: NeuAc2en	Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate	[PMID: 18694948]
HEK293	IC₅₀	43 μM Compound: 1, DANA	Inhibition of human NEU2 expressed in HEK293 cells Inhibition of human NEU2 expressed in HEK293 cells	[PMID: 18068975]
HEK293	IC₅₀	45.5 μM Compound: NeuAc2en	Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate	[PMID: 18694948]
HEK293	IC₅₀	61 μM Compound: 1, DANA	Inhibition of human NEU3 expressed in HEK293 cells Inhibition of human NEU3 expressed in HEK293 cells	[PMID: 18068975]
HEK293	IC₅₀	70.3 μM Compound: NeuAc2en	Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate	[PMID: 18694948]
HEK293	IC₅₀	73.1 μM Compound: NeuAc2en	Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate	[PMID: 18694948]
HEK293	IC₅₀	74 μM Compound: 1, DANA	Inhibition of human NEU4 expressed in HEK293 cells Inhibition of human NEU4 expressed in HEK293 cells	[PMID: 18068975]

In Vitro

N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid (Neu5Ac2en) (10-100 μM) signifcantly inhibits sialidase activity in INS-1D cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid (Neu5Ac2en) (10 mg/kg; i.p.; daily) attenuates pulmonary fibrosis in a mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mice (Mouse model of pulmonary fibrosis)^[3]
Dosage:	10 mg/kg
Administration:	I.p.; daily (starting at day 10 after Bleomycin, and then euthanized at day 21)
Result:	Inhibition of sialidases starting at day 10 after bleomycin attenuates fibrosis.

Clinical Trial

Molecular Weight

291.25

Formula

C₁₁H₁₇NO₈

CAS No.

24967-27-9

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H](O)[C@H]([C@@]1([H])[C@@H]([C@H](C=C(O1)C(O)=O)O)NC(C)=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (114.44 mM; Need ultrasonic)

DMSO : 1 mg/mL (3.43 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4335 mL	17.1674 mL	34.3348 mL
5 mM	0.6867 mL	3.4335 mL	6.8670 mL
10 mM	0.3433 mL	1.7167 mL	3.4335 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Please enter the basic information of animal experiments:

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥99.0%

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Data Sheet (277 KB) SDS (252 KB)

COA (251 KB)

Handling Instructions (2659 KB)

References

[1]. Magesh S, et al. Design, synthesis, and biological evaluation of human sialidase inhibitors. Part 1: selective inhibitors of lysosomal sialidase (NEU1). Bioorg Med Chem Lett. 2008;18(2):532-537. [Content Brief]

[2]. Xiao A, et al. Sialidase-catalyzed one-pot multienzyme (OPME) synthesis of sialidase transition-state analogue inhibitors. ACS Catal. 2018;8(1):43-47. [Content Brief]

[3]. Karhadkar TR, et al. Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model. Sci Rep. 2017;7(1):15069. Published 2017 Nov 8. [Content Brief]

[4]. Minami A, et al. The sialidase inhibitor 2,3-dehydro-2-deoxy-N-acetylneuraminic acid is a glucose-dependent potentiator of insulin secretion. Sci Rep. 2020;10(1):5198. Published 2020 Mar 23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.4335 mL	17.1674 mL	34.3348 mL	85.8369 mL
H₂O	5 mM	0.6867 mL	3.4335 mL	6.8670 mL	17.1674 mL
	10 mM	0.3433 mL	1.7167 mL	3.4335 mL	8.5837 mL
	15 mM	0.2289 mL	1.1445 mL	2.2890 mL	5.7225 mL
	20 mM	0.1717 mL	0.8584 mL	1.7167 mL	4.2918 mL
	25 mM	0.1373 mL	0.6867 mL	1.3734 mL	3.4335 mL
	30 mM	0.1144 mL	0.5722 mL	1.1445 mL	2.8612 mL
	40 mM	0.0858 mL	0.4292 mL	0.8584 mL	2.1459 mL
	50 mM	0.0687 mL	0.3433 mL	0.6867 mL	1.7167 mL
	60 mM	0.0572 mL	0.2861 mL	0.5722 mL	1.4306 mL
	80 mM	0.0429 mL	0.2146 mL	0.4292 mL	1.0730 mL
	100 mM	0.0343 mL	0.1717 mL	0.3433 mL	0.8584 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2,3-Dehydro-2-deoxy-N-acetylneuraminic acid Related Classifications

Infection

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2,3-Dehydro-2-deoxy-N-acetylneuraminic acid24967-27-9Neu5Ac2en DANAInfluenza VirusneuraminidaseenzymespulmonaryfibrosisinsulinsecretionInhibitorinhibitorinhibit

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2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (Synonyms: Neu5Ac2en; DANA)

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2,3-Dehydro-2-deoxy-N-acetylneuraminic acid Related Antibodies

4 Publications Citing Use of MCE 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid

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Complete Stock Solution Preparation Table

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