Cat. No.: HY-W109754

2',4'-Dihydroxylchalcone

(Synonyms: 2',4'-DHC)

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2',4'-Dihydroxychalcone, in combination with nalidixic acid (HY-B0398), exhibits synergistic effects against E. coli by reducing membrane permeability.

For research use only. We do not sell to patients.

Purity: 98.78%
CAS No.: 1776-30-3
Formula: C₁₅H₁₂O₃
Molecular Weight:240.25

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

2',4'-Dihydroxylchalcone Chemical Structure

CAS No.: 1776-30-3

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Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

2',4'-Dihydroxychalcone, in combination with nalidixic acid (HY-B0398), exhibits synergistic effects against E. coli by reducing membrane permeability^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 19	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
A549	IC₅₀	23.2 μM Compound: 8	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 19689125]
A549	IC₅₀	41.6 μM Compound: 27	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 18440233]
B16-BL6	IC₅₀	>100 μM Compound: 8	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 19689125]
B16-BL6	IC₅₀	44.3 μM Compound: 27	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 18440233]
BJ	IC₅₀	262.5 μM Compound: 3a	Cytotoxicity against human BJ cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human BJ cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30482548]
C2C12	IC₅₀	32.26 μg/mL Compound: 12	Cytotoxicity against mouse C2C12 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against mouse C2C12 cells assessed as reduction in cell viability by MTT assay	[PMID: 33412152]
HEK293	IC₅₀	109 μM Compound: 6	Cytotoxicity against human HEK293 cells assessed as cell viability after 24 hrs by cell titer-blue assay Cytotoxicity against human HEK293 cells assessed as cell viability after 24 hrs by cell titer-blue assay	[PMID: 26473275]
HeLa	IC₅₀	23.1 μM Compound: 27	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 18440233]
HeLa	IC₅₀	24.4 μM Compound: 8	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 19689125]
HL-60	IC₅₀	6.3 μM Compound: 3	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 25091929]
HT-1080	IC₅₀	17 μM Compound: 8	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 19689125]
HT-1080	IC₅₀	34 μM Compound: 27	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 18440233]
Lewis lung carcinoma cell line	IC₅₀	39.5 μM Compound: 27	Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay	[PMID: 18440233]
LoVo	IC₅₀	81 μM Compound: 19	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
MCF7	IC₅₀	>10 μM Compound: 2d	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 26690274]
MCF7	IC₅₀	>50 μM Compound: 3a	Antiproliferative activity against human MCF7 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay Antiproliferative activity against human MCF7 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30482548]
MCF7	IC₅₀	16.3 μM Compound: 2b	The compound was tested for antiproliferative activity against MCF-7 human breast cancer cells The compound was tested for antiproliferative activity against MCF-7 human breast cancer cells	[PMID: 11720850]
MDA-MB-231	IC₅₀	>10 μM Compound: 2d	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 26690274]
NALM-6	IC₅₀	18 μM Compound: 3	Cytotoxicity against human NALM6 cells by MTT assay Cytotoxicity against human NALM6 cells by MTT assay	[PMID: 25091929]
PC-3	IC₅₀	>100 μM Compound: 19	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
PC-3	IC₅₀	13.75 μM Compound: 3a	Antiproliferative activity against human PC3 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay Antiproliferative activity against human PC3 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30482548]
PC-3	IC₅₀	8.08 μM Compound: 17a	Cytotoxicity against human PC3 cells assessed as reduction in cell viability Cytotoxicity against human PC3 cells assessed as reduction in cell viability	[PMID: 31539776]
Peritoneal macrophage cell	CC₅₀	416.7 μM Compound: DHC	Cytotoxicity against Swiss albino mouse peritoneal macrophages after 24 hrs by MTT assay Cytotoxicity against Swiss albino mouse peritoneal macrophages after 24 hrs by MTT assay	[PMID: 26169126]
RBL-1	IC₅₀	400 μM Compound: 2	Inhibition of 5-lipoxygenase in rat RBL1 cells Inhibition of 5-lipoxygenase in rat RBL1 cells	10.1007/s00044-013-0745-7
RBL-1	IC₅₀	400 μM Compound: 2	Inhibition of cyclooxygenase in rat RBL1 cells Inhibition of cyclooxygenase in rat RBL1 cells	10.1007/s00044-013-0745-7
RBL-1	IC₅₀	400 μM Compound: 3	In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined	[PMID: 8254620]
SK-MEL-28	IC₅₀	75 μM Compound: 19	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
U-373MG ATCC	IC₅₀	>100 μM Compound: 19	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]
U-937	IC₅₀	80.2 μM Compound: 3	Cytotoxicity against human U937 cells Cytotoxicity against human U937 cells	[PMID: 20035557]
WM-115	IC₅₀	42 μM Compound: 3	Cytotoxicity against human WM115 cells by MTT assay Cytotoxicity against human WM115 cells by MTT assay	[PMID: 25091929]

Chemical Information

CAS No. 1776-30-3
Appearance Solid
Molecular Weight 240.25
Formula C₁₅H₁₂O₃
Color Yellow to orange
SMILES

O=C(C1=CC=C(O)C=C1O)/C=C/C2=CC=CC=C2
Synonyms

2',4'-DHC
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (416.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.1623 mL

20.8117 mL

41.6233 mL

5 mM

0.8325 mL

4.1623 mL

8.3247 mL

10 mM

0.4162 mL

2.0812 mL

4.1623 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (267 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Septama A W, et al. Prospect of plant-based flavonoids to overcome antibacterial resistance: A mini-review. Walailak Journal of Science and Technology (WJST), 2020, 17(5): 503-513.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.1623 mL	20.8117 mL	41.6233 mL	104.0583 mL
	5 mM	0.8325 mL	4.1623 mL	8.3247 mL	20.8117 mL
	10 mM	0.4162 mL	2.0812 mL	4.1623 mL	10.4058 mL
	15 mM	0.2775 mL	1.3874 mL	2.7749 mL	6.9372 mL
	20 mM	0.2081 mL	1.0406 mL	2.0812 mL	5.2029 mL
	25 mM	0.1665 mL	0.8325 mL	1.6649 mL	4.1623 mL
	30 mM	0.1387 mL	0.6937 mL	1.3874 mL	3.4686 mL
	40 mM	0.1041 mL	0.5203 mL	1.0406 mL	2.6015 mL
	50 mM	0.0832 mL	0.4162 mL	0.8325 mL	2.0812 mL
	60 mM	0.0694 mL	0.3469 mL	0.6937 mL	1.7343 mL
	80 mM	0.0520 mL	0.2601 mL	0.5203 mL	1.3007 mL
	100 mM	0.0416 mL	0.2081 mL	0.4162 mL	1.0406 mL

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2',4'-Dihydroxylchalcone

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Biological Activity

Chemical Information

Solvent & Solubility

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References

Help & FAQs

Complete Stock Solution Preparation Table

2',4'-Dihydroxylchalcone

Anti-Infection Compound Library

Antibacterial Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

2',4'-Dihydroxylchalcone Related Classifications

HY-W109754 Related Classifications