2'-Deoxy-2'-fluoro-5-iodouridine
Based on 1 Customer Validation
2'-Deoxy-2'-fluoro-5-iodouridine (FIRU) is a nucleoside analog. When labeled with 123I, 2'-Deoxy-2'-fluoro-5-iodouridine accumulates highly selectively in tumors expressing the HSV1-tk gene. Radiolabeled 2'-Deoxy-2'-fluoro-5-iodouridine enables imaging of adenovirus-mediated HSV1-tk suicide gene transfer.
For research use only. We do not sell to patients.
- Purity: 99.46%
- CAS No.: 55612-21-0
- Formula: C9H10FIN2O5
- Molecular Weight:372.09
-
Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
Anti-Infection Compound Library
HY-L002
-
Cell Cycle/DNA Damage Compound Library
HY-L004
-
Anti-Cancer Compound Library
HY-L025
-
Anti-Aging Compound Library
HY-L034
-
Reprogramming Compound Library
HY-L039
-
Nucleotide Compound Library
HY-L044
-
Anti-Lung Cancer Compound Library
HY-L075
-
Anti-Blood Cancer Compound Library
HY-L079
-
Anti-Cancer Metabolism Compound Library
HY-L083
-
Anti-Hematopathy Compound Library
HY-L171
-
MCE Bioactive Compound Library
HY-L001V
-
High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
|
HSV-1 |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 2.2.15 | EC50 |
>10 μM
Compound: 23
|
Anti hepatitis-B virus activity was evaluated in 2.2.15 cells.
Anti hepatitis-B virus activity was evaluated in 2.2.15 cells.
|
[PMID: 8709113] |
| HepG2 2.2.15 | IC50 |
>200 μM
Compound: 23
|
The growth inhibition activity for the compound was evaluated in 2.2.15 cells.
The growth inhibition activity for the compound was evaluated in 2.2.15 cells.
|
[PMID: 8709113] |
2'-Deoxy-2'-fluoro-5-iodouridine exhibits lower toxicity than FIAU in both HSV1-TK-expressing proliferating cells and HSV1-TK-non-expressing cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 55612-21-0
-
Appearance Solid
-
Molecular Weight 372.09
-
Formula C9H10FIN2O5
-
Color White to off-white
-
SMILES
O[C@H]1[C@@H](F)[C@H](N2C(NC(C(I)=C2)=O)=O)O[C@@H]1CO
-
Synonyms
FIRU
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 87.5 mg/mL (235.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6875 mL | 13.4376 mL | 26.8752 mL | 67.1880 mL |
| 5 mM | 0.5375 mL | 2.6875 mL | 5.3750 mL | 13.4376 mL | |
| 10 mM | 0.2688 mL | 1.3438 mL | 2.6875 mL | 6.7188 mL | |
| 15 mM | 0.1792 mL | 0.8958 mL | 1.7917 mL | 4.4792 mL | |
| 20 mM | 0.1344 mL | 0.6719 mL | 1.3438 mL | 3.3594 mL | |
| 25 mM | 0.1075 mL | 0.5375 mL | 1.0750 mL | 2.6875 mL | |
| 30 mM | 0.0896 mL | 0.4479 mL | 0.8958 mL | 2.2396 mL | |
| 40 mM | 0.0672 mL | 0.3359 mL | 0.6719 mL | 1.6797 mL | |
| 50 mM | 0.0538 mL | 0.2688 mL | 0.5375 mL | 1.3438 mL | |
| 60 mM | 0.0448 mL | 0.2240 mL | 0.4479 mL | 1.1198 mL | |
| 80 mM | 0.0336 mL | 0.1680 mL | 0.3359 mL | 0.8399 mL | |
| 100 mM | 0.0269 mL | 0.1344 mL | 0.2688 mL | 0.6719 mL |
2'-Deoxy-2'-fluoro-5-iodouridine Related Classifications
HY-W342664 Related Classifications
Powered by Bioz