2'-Deoxy-2'-fluorocytidine
Based on 1 publication(s) in Google Scholar
2'-Deoxy-2'-fluorocytidine, an nucleoside analog, is a potent inhibitor of Crimean-Congo hemorrhagic fever virus (CCHFV) replication. 2′-deoxy-2′-fluorocytidine can act synergistically with T705 to increase the potency of both compounds antiviral effects on CCHFV replication.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 10212-20-1
- Formula: C9H12FN3O4
- Molecular Weight:245.21
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
Anti-Infection Compound Library
HY-L002
-
Antiviral Compound Library
HY-L027
-
Nucleotide Compound Library
HY-L044
-
MCE Bioactive Compound Library
HY-L001V
-
High-Throughput Bioactive Compound Library
HY-L205
-
3D Diverse Fragment Library
HY-L903
Publications Citing Use of MedChemExpress (MCE) 2'-Deoxy-2'-fluorocytidine
More
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HEp-2 | EC50 |
>200 μM
Compound: 2'd2'FC
|
Antiviral activity against RSV A2 infected in human Hep2 cells assessed as protection against virus-induced cytopathic effect after 4 days by Cell-Titer Glo assay
Antiviral activity against RSV A2 infected in human Hep2 cells assessed as protection against virus-induced cytopathic effect after 4 days by Cell-Titer Glo assay
|
[PMID: 25978965] |
| HEp-2 | IC50 |
1.8 μM
Compound: 2'd2'FC
|
Inhibition of RSV A2 RNA-dependent RNA polymerase expressed in human Hep2 cells using ATP, GTP, UTP, CTP and 1.5 uCi [alpha-32P] NTP as substrate assessed as reduction of total radiolabled transcript after 90 mins by agarose gel electrophoresis
Inhibition of RSV A2 RNA-dependent RNA polymerase expressed in human Hep2 cells using ATP, GTP, UTP, CTP and 1.5 uCi [alpha-32P] NTP as substrate assessed as reduction of total radiolabled transcript after 90 mins by agarose gel electrophoresis
|
[PMID: 25978965] |
| Huh-7 | CC50 |
>50 μM
Compound: 9
|
Cytotoxicity against human HuH7 cells by MTS assay
Cytotoxicity against human HuH7 cells by MTS assay
|
[PMID: 20580554] |
| MT4 | CC50 |
3.8 μM
Compound: 2'd2'FC
|
Cytotoxicity against human MT4 cells assessed as cell viability after 5 days by Cell-Titer Glo assay
Cytotoxicity against human MT4 cells assessed as cell viability after 5 days by Cell-Titer Glo assay
|
[PMID: 25978965] |
| Vero 76 | CC50 |
>100 μM
Compound: 2'-Fluoro-2'-deoxycytidine
|
Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability by neutral red dye based spectrophotometric analysis
Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability by neutral red dye based spectrophotometric analysis
|
[PMID: 37120998] |
| Vero 76 | CC50 |
>100 μM
Compound: 2'-Fluoro-2'-deoxycytidine
|
Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability by visual analysis
Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability by visual analysis
|
[PMID: 37120998] |
| Vero 76 | CC50 |
249 μM
Compound: 2'-fluoro-2'-deoxycytidine
|
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by neutral red dye based analysis
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by neutral red dye based analysis
|
[PMID: 36898483] |
| Vero 76 | CC50 |
277 μM
Compound: 2'-fluoro-2'-deoxycytidine
|
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by visual analysis
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by visual analysis
|
[PMID: 36898483] |
| Vero 76 | EC50 |
0.7 μM
Compound: 2'-Fluoro-2'-deoxycytidine
|
Antiviral activity against MeV CC strain infected in African green monkey Vero 76 cells assessed as reduction in virus induced cytopathic effect incubated for 6 days by visual analysis
Antiviral activity against MeV CC strain infected in African green monkey Vero 76 cells assessed as reduction in virus induced cytopathic effect incubated for 6 days by visual analysis
|
[PMID: 37120998] |
| Vero 76 | EC50 |
0.95 μM
Compound: 2'-Fluoro-2'-deoxycytidine
|
Antiviral activity against MeV CC strain infected in African green monkey Vero 76 cells assessed as reduction in virus induced cytopathic effect incubated for 6 days by neutral red dye based spectrophotometric analysis
Antiviral activity against MeV CC strain infected in African green monkey Vero 76 cells assessed as reduction in virus induced cytopathic effect incubated for 6 days by neutral red dye based spectrophotometric analysis
|
[PMID: 37120998] |
2'-Deoxy-2'-fluorocytidine has antiviral activities, showing 50% effective concentrations (EC50) of 61 nM and 31 nM against CCHFV and CCHFV/ZsG in Huh7 cells, respectively. And it shows a 50% cytotoxicity concentration (CC50) of >50.0 μM in Huh7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 10212-20-1
-
Appearance Solid
-
Molecular Weight 245.21
-
Formula C9H12FN3O4
-
Color White to yellow
-
SMILES
O[C@H]1[C@@H](F)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Viruses
Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform. [Abstract]2021 Jun 28;13(7):1255. PMID: 34203149
Solvent & Solubility
H2O : 5 mg/mL (20.39 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (407.81 mM); Clear solution; Need ultrasonic
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.0781 mL | 20.3907 mL | 40.7814 mL | 101.9534 mL |
| 5 mM | 0.8156 mL | 4.0781 mL | 8.1563 mL | 20.3907 mL | |
| 10 mM | 0.4078 mL | 2.0391 mL | 4.0781 mL | 10.1953 mL | |
| 15 mM | 0.2719 mL | 1.3594 mL | 2.7188 mL | 6.7969 mL | |
| 20 mM | 0.2039 mL | 1.0195 mL | 2.0391 mL | 5.0977 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
2'-Deoxy-2'-fluorocytidine Related Classifications
HY-W012009 Related Classifications
Powered by Bioz