2-Deoxyribose 5-phosphate disodium

Name: 2-Deoxyribose 5-phosphate disodium
Brand: MedChemExpress
SKU: HY-147061
Rating: 4.5 (1 reviews)

Cat. No.: HY-147061 Purity: 99%: Data Sheet
COA

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Handling Instructions
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2-Deoxyribose 5-phosphate disodium is a substrate of 2-deoxyribose-5-phosphate aldolase (DERA). DERA belongs to class I aldolases, catalyzes reversible aldol reactions, and does not require any cofactors.

For research use only. We do not sell to patients.

2-Deoxyribose 5-phosphate disodium Chemical Structure

CAS No. : 102916-66-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of 2-Deoxyribose 5-phosphate disodium:

2-Deoxyribose 5-phosphate disodium (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs)^[1].
DERA Activity Assay.
1. Prepare 2-Deoxyribose 5-phosphate disodium working solution: Prepare 1 mM 2-Deoxyribose 5-phosphate disodium in 20 mM Na₂HPO₄-citrate buffer (pH 7.0).
2. Experimental Procedures:
2.1 The reaction system contains 20 mM Na₂HPO₄-citrate buffer (pH 7.0), 1 mM substrate (2-Deoxyribose 5-phosphate disodium or 2-deoxyribo), 0.5 mM NADH, 6 U triosephosphate isomerase (EC 5.3.1.1, TPI), 2 U glycerophosphate dehydrogenase (EC 1.1.1.8, GDP) and 10 μL diluted DERA, with a total volume of 200 μL.
2.2 The reaction proceeds at 35 °C for 30 min.
2.3 A universal microplate spectrophotometer is used to measure the decrease in absorbance at 340 nm caused by NADH consumption. The consumption of one NADH molecule corresponds to the decomposition of one DRP molecule. One enzyme unit (U) is defined as the amount of enzyme required to catalyze the cleavage of 1 μmol of substrate per minute under the assay conditions.
2.4 Protein concentration is quantified using a BCA Protein Assay Kit according to the method reported in the literature.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

258.07

Formula

C₅H₉Na₂O₇P

CAS No.

102916-66-5

Appearance

Solid

Color

White to off-white

SMILES

O=CC[C@H](O)[C@H](O)COP(O[Na])(O[Na])=O

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (387.49 mM; ultrasonic and adjust pH to 5 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 83.33 mg/mL (322.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8749 mL	19.3746 mL	38.7492 mL
5 mM	0.7750 mL	3.8749 mL	7.7498 mL
10 mM	0.3875 mL	1.9375 mL	3.8749 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99%

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Data Sheet (272 KB) SDS (252 KB)

COA (248 KB)

Handling Instructions (2659 KB)

References

[1]. You ZY, et al. Characterization and application of a newly synthesized 2-deoxyribose-5-phosphate aldolase. J Ind Microbiol Biotechnol. 2013;40(1):29-39. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.8749 mL	19.3746 mL	38.7492 mL	96.8729 mL
	5 mM	0.7750 mL	3.8749 mL	7.7498 mL	19.3746 mL
	10 mM	0.3875 mL	1.9375 mL	3.8749 mL	9.6873 mL
	15 mM	0.2583 mL	1.2916 mL	2.5833 mL	6.4582 mL
	20 mM	0.1937 mL	0.9687 mL	1.9375 mL	4.8436 mL
	25 mM	0.1550 mL	0.7750 mL	1.5500 mL	3.8749 mL
	30 mM	0.1292 mL	0.6458 mL	1.2916 mL	3.2291 mL
	40 mM	0.0969 mL	0.4844 mL	0.9687 mL	2.4218 mL
	50 mM	0.0775 mL	0.3875 mL	0.7750 mL	1.9375 mL
	60 mM	0.0646 mL	0.3229 mL	0.6458 mL	1.6145 mL
	80 mM	0.0484 mL	0.2422 mL	0.4844 mL	1.2109 mL
	100 mM	0.0387 mL	0.1937 mL	0.3875 mL	0.9687 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.