2-Deoxyribose 5-phosphate disodium
Based on 1 Customer Validation
2-Deoxyribose 5-phosphate disodium is a substrate of 2-deoxyribose-5-phosphate aldolase (DERA). DERA belongs to class I aldolases, catalyzes reversible aldol reactions, and does not require any cofactors.
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- Pureza: 99%
- No. CAS: 102916-66-5
- Fòrmula: C5H9Na2O7P
- Peso molecular:258.07
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Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs)[1].
DERA Activity Assay.
1. Prepare 2-Deoxyribose 5-phosphate disodium working solution: Prepare 1 mM 2-Deoxyribose 5-phosphate disodium in 20 mM Na2HPO4-citrate buffer (pH 7.0).
2. Experimental Procedures:
2.1 The reaction system contains 20 mM Na2HPO4-citrate buffer (pH 7.0), 1 mM substrate (2-Deoxyribose 5-phosphate disodium or 2-deoxyribo), 0.5 mM NADH, 6 U triosephosphate isomerase (EC 5.3.1.1, TPI), 2 U glycerophosphate dehydrogenase (EC 1.1.1.8, GDP) and 10 μL diluted DERA, with a total volume of 200 μL.
2.2 The reaction proceeds at 35 °C for 30 min.
2.3 A universal microplate spectrophotometer is used to measure the decrease in absorbance at 340 nm caused by NADH consumption. The consumption of one NADH molecule corresponds to the decomposition of one DRP molecule. One enzyme unit (U) is defined as the amount of enzyme required to catalyze the cleavage of 1 μmol of substrate per minute under the assay conditions.
2.4 Protein concentration is quantified using a BCA Protein Assay Kit according to the method reported in the literature.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 102916-66-5
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Appearance Solid
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Peso molecular 258.07
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Fòrmula C5H9Na2O7P
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Color White to off-white
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SMILES
O=CC[C@H](O)[C@H](O)COP(O[Na])(O[Na])=O
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
DMSO : 100 mg/mL (387.49 mM; ultrasonic and adjust pH to 5 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 83.33 mg/mL (322.90 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.8749 mL | 19.3746 mL | 38.7492 mL | 96.8729 mL |
| 5 mM | 0.7750 mL | 3.8749 mL | 7.7498 mL | 19.3746 mL | |
| 10 mM | 0.3875 mL | 1.9375 mL | 3.8749 mL | 9.6873 mL | |
| 15 mM | 0.2583 mL | 1.2916 mL | 2.5833 mL | 6.4582 mL | |
| 20 mM | 0.1937 mL | 0.9687 mL | 1.9375 mL | 4.8436 mL | |
| 25 mM | 0.1550 mL | 0.7750 mL | 1.5500 mL | 3.8749 mL | |
| 30 mM | 0.1292 mL | 0.6458 mL | 1.2916 mL | 3.2291 mL | |
| 40 mM | 0.0969 mL | 0.4844 mL | 0.9687 mL | 2.4218 mL | |
| 50 mM | 0.0775 mL | 0.3875 mL | 0.7750 mL | 1.9375 mL | |
| 60 mM | 0.0646 mL | 0.3229 mL | 0.6458 mL | 1.6145 mL | |
| 80 mM | 0.0484 mL | 0.2422 mL | 0.4844 mL | 1.2109 mL | |
| 100 mM | 0.0387 mL | 0.1937 mL | 0.3875 mL | 0.9687 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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