3-Azido-7-hydroxycoumarin
Based on 1 Customer Validation
3-Azido-7-hydroxycoumarin is a click chemistry reagent containing an azido group. Under Cu (II) catalysis, it undergoes azide-alkyne cycloaddition (CuAAC) click reaction with 3-butyn-1-ol and emits a fluorescent signal.
For research use only. We do not sell to patients.
- Purity: 99.68%
- CAS No.: 817638-68-9
- Formula: C9H5N3O3
- Molecular Weight:203.15
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
41.62 μM
Compound: 2a
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Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
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[PMID: 27769619] |
| MCF7 | IC50 |
38.4 μM
Compound: 2a
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Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
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[PMID: 27769619] |
| MRC5 | IC50 |
62.7 μM
Compound: 2a
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Cytotoxicity against human MRC5 cells after 24 hrs by MTT assay
Cytotoxicity against human MRC5 cells after 24 hrs by MTT assay
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[PMID: 27769619] |
3-Azido-7-hydroxycoumarin (1 mM; 15 min) serves as a substrate for the CuAAC click reaction catalyzed by Phage@DNAzyme, generating a fluorescent signal to enable the detection of Escherichia coli and Salmonella typhimurium[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 817638-68-9
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Appearance Solid
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Molecular Weight 203.15
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Formula C9H5N3O3
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Color Yellow to brown
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SMILES
OC1=CC(OC(C(N=[N+]=[N-])=C2)=O)=C2C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (246.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (585 KB)
- English - EN (585 KB)
- Français - FR (585 KB)
- Deutsch - DE (585 KB)
- Norwegian - NO (585 KB)
- Español - ES (585 KB)
- Swedish - SV (585 KB)
- Italian - IT (585 KB)
- Korean - KR (585 KB)
- Portuguese - PT (585 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.9225 mL | 24.6124 mL | 49.2247 mL | 123.0618 mL |
| 5 mM | 0.9845 mL | 4.9225 mL | 9.8449 mL | 24.6124 mL | |
| 10 mM | 0.4922 mL | 2.4612 mL | 4.9225 mL | 12.3062 mL | |
| 15 mM | 0.3282 mL | 1.6408 mL | 3.2816 mL | 8.2041 mL | |
| 20 mM | 0.2461 mL | 1.2306 mL | 2.4612 mL | 6.1531 mL | |
| 25 mM | 0.1969 mL | 0.9845 mL | 1.9690 mL | 4.9225 mL | |
| 30 mM | 0.1641 mL | 0.8204 mL | 1.6408 mL | 4.1021 mL | |
| 40 mM | 0.1231 mL | 0.6153 mL | 1.2306 mL | 3.0765 mL | |
| 50 mM | 0.0984 mL | 0.4922 mL | 0.9845 mL | 2.4612 mL | |
| 60 mM | 0.0820 mL | 0.4102 mL | 0.8204 mL | 2.0510 mL | |
| 80 mM | 0.0615 mL | 0.3077 mL | 0.6153 mL | 1.5383 mL | |
| 100 mM | 0.0492 mL | 0.2461 mL | 0.4922 mL | 1.2306 mL |
3-Azido-7-hydroxycoumarin Related Classifications
HY-W540978 Related Classifications
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