3-Hydroxyphenylacetic acid
Based on 1 publication(s) in Google Scholar
3-Hydroxyphenylacetic acid is an orally active flavonoid metabolite produced by intestinal flora, with blood pressure-lowering activity. 3-Hydroxyphenylacetic acid can also inhibit ferroptosis by upregulating the expression of GPX4, thereby improving spermatogenic dysfunction in aged mice. In addition, abnormal levels of 3-Hydroxyphenylacetic acid are closely related to certain diseases, such as autism spectrum disorders.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.03%
- No. CAS: 621-37-4
- Fòrmula: C8H8O3
- Peso molecular:152.15
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
HY-N1902
4-Hydroxyphenylacetic acid -
HY-W001080
3,4-Dihydroxybenzeneacetic acid -
HY-W015590
2-Hydroxyphenylacetic acid -
HY-113219
Hydroxyphenyllactic acid -
HY-N0593
Deoxycholic acid -
HY-W016562
Hippuric acid -
HY-N2406
Dihydrocaffeic acid -
HY-B0430
D-Pantothenic acid -
HY-B2123
Lactose -
HY-W015346
Desaminotyrosine -
HY-W001083R
3-Hydroxyphenylacetic acid (Standard) -
HY-N1902R
4-Hydroxyphenylacetic acid (Standard)
Publications Citing Use of MedChemExpress (MCE) 3-Hydroxyphenylacetic acid
MoreVer todos los productos específicos de isoformas Endogenous Metabolite
More
Actividad biológica
Descripciòn
IC50 & Target
|
GPX4 |
In Vitro
3-Hydroxyphenylacetic acid (0-50 μM; 6-9 h) can enhance total ALDH activity and completely inhibit acetaldehyde-induced cytotoxicity in mouse hepatocellular carcinoma Hepa1c1c7 cells[1].
3-Hydroxyphenylacetic acid (0.1-1 mM) can induce dose-dependent vasodilation in porcine coronary artery segments[2].
3-Hydroxyphenylacetic acid (50 μM; 48 h) can inhibit cell apoptosis, reduce ROS production, decrease MDA levels, increase GPx activity, and upregulate GPX4 protein expression in H2O2-induced senescent GC-2 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:GC-2 cells treated H2O2
-
Concentration:50 μM
-
Incubation Time:48 h
-
Result:Significantly increased GPX4 protein expression, although no change was found for FTL, ACSL4, and xCT.
In Vivo
3-Hydroxyphenylacetic acid (25 mg/kg; oral gavage; 6 weeks) can improve spermatogenic dysfunction in aged mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Spontaneously hypertensive rats (SHR) (average weight 356 ± 21 g)[2]
-
Dosage:0.001, 0.01. 0.1, 0.5, 1, 2.5, 5 and 10 mg/kg (the volume applied was always 0.1 mL)
-
Administration:Intravenous bolus; single dose
-
Result:Decreased the systolic, diastolic, and mean arterial pressures of SHR in a dose-dependent manner, with 0.01 mg/kg significantly reducing diastolic pressure and 10 mg/kg showing the most significant antihypertensive effect.
Had no significant effect on heart rate.
-
Animal Model:Male C57BL/6J mice aged 18-20 months old[3]
-
Dosage:25 mg/kg
-
Administration:Oral gavage; 6 weeks
-
Result:Increased sperm concentration.
Improved spermatogenesis with better-organized seminiferous tubules and increased numbers of spermatogonia, spermatocytes, spermatids, and spermatozoa.
Increased the expression of GPX4 and NRF2.
Increased total GPx activity, level of GSH, and ratio of GSH/GSSG, along with decreased MDA abundance, indicating restrained ferroptosis.
Chemical Information
-
No. CAS 621-37-4
-
Appearance Solid
-
Peso molecular 152.15
-
Fòrmula C8H8O3
-
Color White to off-white
-
SMILES
OC1=CC=CC(CC(O)=O)=C1
-
Structure Classification
-
Initial Source
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Microbiome
The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis. [Abstract]2023 Sep 27;11(1):212. PMID: 37752615
Solvente y solubilidad
In Vitro:
DMSO : 50 mg/mL (328.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (164.31 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureza y Documentación
-
Ficha de datos (288 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
[1]. Liu Y, et al. A Major Intestinal Catabolite of Quercetin Glycosides, 3-Hydroxyphenylacetic Acid, Protects the Hepatocytes from the Acetaldehyde-Induced Cytotoxicity through the Enhancement of the Total Aldehyde Dehydrogenase Activity. Int J Mol Sci. 2022 Feb 3;23(3):1762. [Content Brief]
[2]. Dias P, et al. 3-Hydroxyphenylacetic Acid: A Blood Pressure-Reducing Flavonoid Metabolite. Nutrients. 2022 Jan 13;14(2):328. [Content Brief]
[3]. Jin Z, et al. The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis. Microbiome. 2023 Sep 27;11(1):212. [Content Brief]
[4]. Xiong X, et al. Urinary 3-(3-Hydroxyphenyl)-3-hydroxypropionic Acid, 3-Hydroxyphenylacetic Acid, and 3-Hydroxyhippuric Acid Are Elevated in Children with Autism Spectrum Disorders. Biomed Res Int. 2016;2016:9485412. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 6.5725 mL | 32.8623 mL | 65.7246 mL | 164.3115 mL |
| 5 mM | 1.3145 mL | 6.5725 mL | 13.1449 mL | 32.8623 mL | |
| 10 mM | 0.6572 mL | 3.2862 mL | 6.5725 mL | 16.4312 mL | |
| 15 mM | 0.4382 mL | 2.1908 mL | 4.3816 mL | 10.9541 mL | |
| 20 mM | 0.3286 mL | 1.6431 mL | 3.2862 mL | 8.2156 mL | |
| 25 mM | 0.2629 mL | 1.3145 mL | 2.6290 mL | 6.5725 mL | |
| 30 mM | 0.2191 mL | 1.0954 mL | 2.1908 mL | 5.4771 mL | |
| 40 mM | 0.1643 mL | 0.8216 mL | 1.6431 mL | 4.1078 mL | |
| 50 mM | 0.1314 mL | 0.6572 mL | 1.3145 mL | 3.2862 mL | |
| 60 mM | 0.1095 mL | 0.5477 mL | 1.0954 mL | 2.7385 mL | |
| 80 mM | 0.0822 mL | 0.4108 mL | 0.8216 mL | 2.0539 mL | |
| 100 mM | 0.0657 mL | 0.3286 mL | 0.6572 mL | 1.6431 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
3-Hydroxyphenylacetic acid Related Classifications
HY-W001083 Related Classifications
Powered by Bioz