3-(N-Maleimidopropionyl)biocytin
Based on 1 Customer Validation
3-(N-Maleimidopropionyl)biocytin is a cysteine-specific labeling reagent and non-membrane-permeable probe. 3-(N-Maleimidopropionyl)biocytin covalently modifies the exposed cysteine residues of yeast Tim44, conjugating the biotin moiety to the polypeptide chain. 3-(N-Maleimidopropionyl)biocytin determines the topology of membrane proteins by modifying the exposed cysteine residues on the outer side of the inner mitochondrial membrane.
For research use only. We do not sell to patients.
- Purity : 85.2%
- CAS No.: 102849-12-7
- Formula: C23H33N5O7S
- Molecular Weight:523.60
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
3-(N-Maleimidopropionyl)biocytin (0.5 mM; 30 min at 4 °C) labels the single cysteine residue (Cys-369) of yeast Tim44 in intact mitochondria, demonstrating that the C-terminal domain of yeast Tim44 is exposed to the mitochondrial intermembrane space[1].
3-(N-Maleimidopropionyl)biocytin (0.5 mM; 30 min at 4 °C) does not label rat liver Tim44 in either intact or solubilized mitochondria, demonstrating that all cysteine residues of rat liver Tim44 are inaccessible to the reagent[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 102849-12-7
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Appearance Solid
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Molecular Weight 523.60
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Formula C23H33N5O7S
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Color White to light yellow
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SMILES
O=C(C=CC1=O)N1CCC(N[C@H](C(O)=O)CCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 12.5 mg/mL (23.87 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9099 mL | 9.5493 mL | 19.0985 mL | 47.7464 mL |
| 5 mM | 0.3820 mL | 1.9099 mL | 3.8197 mL | 9.5493 mL | |
| 10 mM | 0.1910 mL | 0.9549 mL | 1.9099 mL | 4.7746 mL | |
| 15 mM | 0.1273 mL | 0.6366 mL | 1.2732 mL | 3.1831 mL | |
| 20 mM | 0.0955 mL | 0.4775 mL | 0.9549 mL | 2.3873 mL |
3-(N-Maleimidopropionyl)biocytin Related Classifications
HY-W020784 Related Classifications
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