3-(N-Maleimidopropionyl)biocytin 

3-(N-Maleimidopropionyl)biocytin is a cysteine-specific labeling reagent and non-membrane-permeable probe. 3-(N-Maleimidopropionyl)biocytin covalently modifies the exposed cysteine residues of yeast Tim44, conjugating the biotin moiety to the polypeptide chain. 3-(N-Maleimidopropionyl)biocytin determines the topology of membrane proteins by modifying the exposed cysteine residues on the outer side of the inner mitochondrial membrane^[1].

3-(N-Maleimidopropionyl)biocytin (0.5 mM; 30 min at 4 °C) labels the single cysteine residue (Cys-369) of yeast Tim44 in intact mitochondria, demonstrating that the C-terminal domain of yeast Tim44 is exposed to the mitochondrial intermembrane space^[1].
3-(N-Maleimidopropionyl)biocytin (0.5 mM; 30 min at 4 °C) does not label rat liver Tim44 in either intact or solubilized mitochondria, demonstrating that all cysteine residues of rat liver Tim44 are inaccessible to the reagent^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

523.60

C₂₃H₃₃N₅O₇S

102849-12-7

Solid

White to light yellow

O=C(C=CC1=O)N1CCC(N[C@H](C(O)=O)CCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Pavlov PF, et al. Probing the membrane topology of a subunit of the mitochondrial protein translocase, Tim44, with biotin maleimide. Biochem Biophys Res Commun. 2002;293(1):321-326.  [Content Brief]