3-Nitrophenylhydrazine-¹³C₆ hydrochloride

Name: 3-Nitrophenylhydrazine- 13 C 6 hydrochloride
Brand: MedChemExpress
SKU: HY-133689AS
Rating: 4.5 (1 reviews)

Cat. No.: HY-133689AS Purity: 98.26%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

3-Nitrophenylhydrazine-¹³C₆ (hydrochloride) is the ¹³C labeled 3-Nitrophenylhydrazine hydrochloride[1].

For research use only. We do not sell to patients.

3-Nitrophenylhydrazine-<sup>13</sup>C<sub>6</sub> hydrochloride Chemical Structure

3-Nitrophenylhydrazine-¹³C₆ hydrochloride Chemical Structure

CAS No. : 1977535-33-3

Size	Price	Stock	Quantity
1 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

3-Nitrophenylhydrazine-¹³C₆ (hydrochloride) is the ¹³C labeled 3-Nitrophenylhydrazine hydrochloride[1].

In Vitro

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

195.56

Formula

¹³C₆H₈ClN₃O₂

CAS No.

1977535-33-3

Appearance

Solid

Color

White to light yellow

SMILES

NN[13C]1=[13CH][13CH]=[13CH][13C]([N+]([O-])=O)=[13CH]1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (255.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1135 mL	25.5676 mL	51.1352 mL
5 mM	1.0227 mL	5.1135 mL	10.2270 mL
10 mM	0.5114 mL	2.5568 mL	5.1135 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.25 mg/mL (6.39 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (6.39 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.26%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (252 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.1135 mL	25.5676 mL	51.1352 mL	127.8380 mL
	5 mM	1.0227 mL	5.1135 mL	10.2270 mL	25.5676 mL
	10 mM	0.5114 mL	2.5568 mL	5.1135 mL	12.7838 mL
	15 mM	0.3409 mL	1.7045 mL	3.4090 mL	8.5225 mL
	20 mM	0.2557 mL	1.2784 mL	2.5568 mL	6.3919 mL
	25 mM	0.2045 mL	1.0227 mL	2.0454 mL	5.1135 mL
	30 mM	0.1705 mL	0.8523 mL	1.7045 mL	4.2613 mL
	40 mM	0.1278 mL	0.6392 mL	1.2784 mL	3.1960 mL
	50 mM	0.1023 mL	0.5114 mL	1.0227 mL	2.5568 mL
	60 mM	0.0852 mL	0.4261 mL	0.8523 mL	2.1306 mL
	80 mM	0.0639 mL	0.3196 mL	0.6392 mL	1.5980 mL
	100 mM	0.0511 mL	0.2557 mL	0.5114 mL	1.2784 mL