3'-ONH2-dATP sodium solution (100 mM) 

3'-ONH2-dATP (sodium) solution (100 mM) is a 3'-O-blocked reversible terminator deoxynucleotide triphosphate.3'-ONH2-dATP (sodium) solution (100 mM) stops DNA polymerization after single-nucleotide addition to an initiator strand, and its 3'-ONH2 blocking group can be removed to restore a free 3'-OH for subsequent extension.3'-ONH2-dATP (sodium) solution (100 mM) incorporates into an oligonucleotide chain by engineered terminal deoxynucleotidyl transferase from Zonotrichia albicollis to enable template-free, stepwise de novo enzymatic DNA synthesis^[1].

3'-ONH2-dATP (0.25 mM; 20 min) led to wild-type ZaTdT exhibiting negligible catalytic activity for 3'-ONH₂-dATP, with no detectable incorporation into CT-terminated synthetic DNA initiator strands under tested conditions^[1].
3'-ONH2-dATP (0.25 mM; 20 min) led to engineered ZaTdT-R335A exhibiting 20-fold higher catalytic activity for 3'-ONH₂-dATP than wild-type ZaTdT when tested with synthetic DNA initiator strands^[1].
3'-ONH2-dATP (0.25 mM; 20 min) led to engineered ZaTdT^R335L exhibiting a 2.6-fold higher catalytic activity for 3'-ONH₂-dATP than ZaTdT^R335A when tested with synthetic DNA initiator strands^[1].
3'-ONH2-dATP (10 min per cycle) led to engineered ZaTdT^R335L-K337G enabling efficient de novo enzymatic DNA synthesis with 3'-ONH₂-dATP, supporting an average stepwise yield of 98.7% across 10 cycles and an 87.7% yield of full-length target synthetic DNA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C₁₀H₁₇N₆O₁₂P₃.xNa

Liquid

Colorless to light yellow

O=P(O)(O)OP(OP(OC[C@H]1O[C@@H](N2C(N=CN=C3N)=C3N=C2)C[C@@H]1ON)(O)=O)(O)=O.[Na].[x]

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Xiaoyun Lu, et al. Enzymatic DNA Synthesis by Engineering Terminal Deoxynucleotidyl Transferase. ACS Catalysis.February 18, 2022.