4-Methylumbelliferyl butyrate
Based on 1 Customer Validation
4-Methylumbelliferyl butyrate (4-MUB) is a fluorogenic esterase substrate that is hydrolyzed by esterases to release the fluorescent product 4-methylumbelliferone (HY-N0187). 4-Methylumbelliferyl butyrate is hydrolyzed by the general esterases (GE) of Daphnia magna and Chironomus riparius; it also serves as a substrate for the recombinant mycobacterial carboxylesterase MT2282 (CaeA). 4-Methylumbelliferyl butyrate is used to determine the activity of the S. aureus serine hydrolases FphB and FphH. 4-Methylumbelliferyl butyrate is used in studies related to esterase activity assays.
For research use only. We do not sell to patients.
- Purity : 99.01%
- CAS No.: 17695-46-4
- Formula: C14H14O4
- Molecular Weight:246.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
4-Methylumbelliferyl butyrate (4-MUB) is used as a substrate for general esterase (GE) inhibition assays; with 4-MUB as the substrate, Iso-OMPA inhibits general esterase in D. magna with an IC50 = 63.3 μM, and inhibits general esterase in C. riparius larvae with an IC50 = 447 μM[1].
4-methylumbelliferyl butyrate is efficiently cleaved by recombinant FphB and FphH, and thus serves as a preferred fluorescent screening substrate in high-throughput screening assays for covalent inhibitors of FphB and FphH[2].
4-Methylumbelliferyl butyrate (4-MUB) is preferentially hydrolyzed by purified recombinant MT2282, with the corresponding kinetic parameters of Vmax = 128.1 relative fluorescence units/min and Km = 738 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 17695-46-4
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Appearance Solid
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Molecular Weight 246.26
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Formula C14H14O4
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Color White to off-white
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SMILES
CCCC(OC1=CC=C(C(O2)=C1)C(C)=CC2=O)=O
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Synonyms
4-MUB
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (406.07 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0607 mL | 20.3037 mL | 40.6075 mL | 101.5187 mL |
| 5 mM | 0.8121 mL | 4.0607 mL | 8.1215 mL | 20.3037 mL | |
| 10 mM | 0.4061 mL | 2.0304 mL | 4.0607 mL | 10.1519 mL | |
| 15 mM | 0.2707 mL | 1.3536 mL | 2.7072 mL | 6.7679 mL | |
| 20 mM | 0.2030 mL | 1.0152 mL | 2.0304 mL | 5.0759 mL | |
| 25 mM | 0.1624 mL | 0.8121 mL | 1.6243 mL | 4.0607 mL | |
| 30 mM | 0.1354 mL | 0.6768 mL | 1.3536 mL | 3.3840 mL | |
| 40 mM | 0.1015 mL | 0.5076 mL | 1.0152 mL | 2.5380 mL | |
| 50 mM | 0.0812 mL | 0.4061 mL | 0.8121 mL | 2.0304 mL | |
| 60 mM | 0.0677 mL | 0.3384 mL | 0.6768 mL | 1.6920 mL | |
| 80 mM | 0.0508 mL | 0.2538 mL | 0.5076 mL | 1.2690 mL | |
| 100 mM | 0.0406 mL | 0.2030 mL | 0.4061 mL | 1.0152 mL |
4-Methylumbelliferyl butyrate Related Classifications
HY-135036 Related Classifications
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Keywords
- 4-Methylumbelliferyl butyrate
- 17695-46-4
- 4-MUB
- Fluorescent Dye
- Drug Intermediate
- rice bran extracts
- HEK293 cells
- Chironomus riparius
- S. aureus serine hydrolases FphB and FphH
- recombinant OsEST-b
- MT2282 carboxylesterase
- Ser215-Asp450-His477 catalytic triad
- E. coli Tuner(DE3)pLysS
- Daphnia magna
- mfCES2C
- Inhibitor
- inhibitor
- inhibit