5-Azidopentanoic acid ethyl ester
5-Azidopentanoic acid ethyl ester is an azide compound that can be used to synthesize other compounds. 5-Azidopentanoic acid ethyl ester has been shown to inhibit the proliferation of cancer cells in vitro and in vivo. 5-Azidopentanoic acid ethyl ester reacts with amines to form a tethered compound, which can then be used as a probe for identifying amine-containing proteins.
For research use only. We do not sell to patients.
- CAS No.: 89896-39-9
- Formula: C7H13N3O2
- Molecular Weight:171.20
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Chemical Information
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CAS No. 89896-39-9
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Molecular Weight 171.20
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Formula C7H13N3O2
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SMILES
[N-]=[N+]=NCCCCC(OCC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)