5'-O-DMT-N2-ibu-dG
Based on 1 Customer Validation
5'-O-DMT-N2-ibu-dG (N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine) is a protected 2'-deoxyguanosine derivative with 5'-O-(4,4'-dimethoxytriphenylmethyl) and N2-isobutyryl protecting groups, and serves as a building block in oligonucleotide synthesis.
For research use only. We do not sell to patients.
- Purity: 99.26%
- CAS No.: 68892-41-1
- Formula: C35H37N5O7
- Molecular Weight:639.70
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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HY-L004
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HY-L044
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Biological Activity
5'-O-DMT-N2-ibu-dG is a crucial protective nucleotide building block in DNA synthesis. 5'-O-DMT-N2-ibu-dG serves as a DNA monomer raw material in solid-phase phosphoramidite triester synthesis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 68892-41-1
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Appearance Solid
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Molecular Weight 639.70
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Formula C35H37N5O7
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Color White to off-white
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SMILES
O[C@H]1C[C@H](N2C(N=C(NC(C(C)C)=O)NC3=O)=C3N=C2)O[C@@H]1COC(C4=CC=C(OC)C=C4)(C5=CC=C(OC)C=C5)C6=CC=CC=C6
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Synonyms
N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (156.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5632 mL | 7.8162 mL | 15.6323 mL | 39.0808 mL |
| 5 mM | 0.3126 mL | 1.5632 mL | 3.1265 mL | 7.8162 mL | |
| 10 mM | 0.1563 mL | 0.7816 mL | 1.5632 mL | 3.9081 mL | |
| 15 mM | 0.1042 mL | 0.5211 mL | 1.0422 mL | 2.6054 mL | |
| 20 mM | 0.0782 mL | 0.3908 mL | 0.7816 mL | 1.9540 mL | |
| 25 mM | 0.0625 mL | 0.3126 mL | 0.6253 mL | 1.5632 mL | |
| 30 mM | 0.0521 mL | 0.2605 mL | 0.5211 mL | 1.3027 mL | |
| 40 mM | 0.0391 mL | 0.1954 mL | 0.3908 mL | 0.9770 mL | |
| 50 mM | 0.0313 mL | 0.1563 mL | 0.3126 mL | 0.7816 mL | |
| 60 mM | 0.0261 mL | 0.1303 mL | 0.2605 mL | 0.6513 mL | |
| 80 mM | 0.0195 mL | 0.0977 mL | 0.1954 mL | 0.4885 mL | |
| 100 mM | 0.0156 mL | 0.0782 mL | 0.1563 mL | 0.3908 mL |
5'-O-DMT-N2-ibu-dG Related Classifications
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