5'-ODMT cEt m5U Phosphoramidite (Amidite)
Based on 1 Customer Validation
5'-ODMT cEt m5U Phosphoramidite Amidite is a locked nucleic acid (LNA) analog. 5'-ODMT cEt m5U Phosphoramidite Amidite shows excellent safety and antisense activity in mice.
For research use only. We do not sell to patients.
- Purity: 99.76%
- CAS No.: 1197033-22-9
- Formula: C42H51N4O9P
- Molecular Weight:786.85
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Storage:
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
5′-methyl DNA modified ASOs exhibit excellent antisense activity[2].
5'-ODMT cEt m5U Phosphoramidite Amidite (Compound 74) can be synthesized from the thymine-modified S-cEt nucleoside 62[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1197033-22-9
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Appearance Solid
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Molecular Weight 786.85
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Formula C42H51N4O9P
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Color White to off-white
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SMILES
COC1=CC=C(C(C2=CC=C(OC)C=C2)(OC[C@]3(O[C@H]4N5C=C(C)C(NC5=O)=O)[C@@H](O[C@@]4([C@@H]3OP(N(C(C)C)C(C)C)OCCC#N)[H])C)C6=CC=CC=C6)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (158.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Vasquez G, et al. Site-specific incorporation of 5'-methyl DNA enhances the therapeutic profile of gapmer ASOs. Nucleic Acids Res. 2021 Feb 26;49(4):1828-1839. [Content Brief]
[3]. Seth PP, et al. Synthesis and biophysical evaluation of 2',4'-constrained 2'O-methoxyethyl and 2',4'-constrained 2'O-ethyl nucleic acid analogues. J Org Chem. 2010 Mar 5;75(5):1569-81. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2709 mL | 6.3545 mL | 12.7089 mL | 31.7723 mL |
| 5 mM | 0.2542 mL | 1.2709 mL | 2.5418 mL | 6.3545 mL | |
| 10 mM | 0.1271 mL | 0.6354 mL | 1.2709 mL | 3.1772 mL | |
| 15 mM | 0.0847 mL | 0.4236 mL | 0.8473 mL | 2.1182 mL | |
| 20 mM | 0.0635 mL | 0.3177 mL | 0.6354 mL | 1.5886 mL | |
| 25 mM | 0.0508 mL | 0.2542 mL | 0.5084 mL | 1.2709 mL | |
| 30 mM | 0.0424 mL | 0.2118 mL | 0.4236 mL | 1.0591 mL | |
| 40 mM | 0.0318 mL | 0.1589 mL | 0.3177 mL | 0.7943 mL | |
| 50 mM | 0.0254 mL | 0.1271 mL | 0.2542 mL | 0.6354 mL | |
| 60 mM | 0.0212 mL | 0.1059 mL | 0.2118 mL | 0.5295 mL | |
| 80 mM | 0.0159 mL | 0.0794 mL | 0.1589 mL | 0.3972 mL | |
| 100 mM | 0.0127 mL | 0.0635 mL | 0.1271 mL | 0.3177 mL |
5'-ODMT cEt m5U Phosphoramidite (Amidite) Related Classifications
HY-148506 Related Classifications
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