5(6)-FAM SE (Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers)

Cat. No.: HY-15937 Purity: 95.42%: FAQs
Data Sheet SDS COA Handling Instructions

5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

For research use only. We do not sell to patients.

5(6)-FAM SE Chemical Structure

CAS No. : 117548-22-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 144	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

Molecular Weight

473.39

Appearance

Solid

Formula

C₂₅H₁₅NO₉

CAS No.

117548-22-8

Emission (Em)

525

Excitation (Ex)

488

SMILES

OC1=CC=C2C(OC(C(C=C3)=C2C(C=CC(C(ON(C(CC4)=O)C4=O)=O)=C5)=C5C(O)=O)=CC3=O)=C1.OC6=CC=C7C(OC(C(C=C8)=C7C(C=C(C(ON(C(CC9)=O)C9=O)=O)C=C%10)=C%10C(O)=O)=CC8=O)=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (176.03 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1124 mL	10.5621 mL	21.1242 mL
5 mM	0.4225 mL	2.1124 mL	4.2248 mL
10 mM	0.2112 mL	1.0562 mL	2.1124 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 6.25 mg/mL (13.20 mM); Suspended solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (4.39 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: 95.42%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (256 KB) HNMR (189 KB) LCMS (401 KB)

Handling Instructions (2659 KB)

References

[1]. Stafford JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter. J Biol Chem. 2001 Oct 26;276(43):39885-91. [Content Brief]

[2]. Powell RD, et al. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc Res Tech. 1998 Jul 1;42(1):2-12. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

5(6)-FAM SE117548-22-85(6)-Carboxyfluorescein N-hydroxysuccinimide ester 5(6)-Carboxyfluorescein succinimidyl ester mixed isomersFluorescent DyeInhibitorinhibitorinhibit

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5(6)-FAM SE (Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers)

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator