5(6)-FAM SE
Based on 4 publication(s) in Google Scholar
5(6)-FAM SE is a green fluorescent dye widely used for protein labeling.
For research use only. We do not sell to patients.
- Purity: 95.76%
- CAS No.: 117548-22-8
- Formula: C25H15NO9
- Molecular Weight:473.39
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5(6)-FAM SE
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Biological Activity
5(6)-FAM SE labels proteins, which decreases exponentially during the cell division, and corresponds to the cell proliferation degree. 5(6)-FAM SE can be used to assess the cell proliferation degree and cell cycle status[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 117548-22-8
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Appearance Solid
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Molecular Weight 473.39
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Formula C25H15NO9
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Color Yellow to orange
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SMILES
OC1=CC=C2C(OC(C(C=C3)=C2C(C=CC(C(ON(C(CC4)=O)C4=O)=O)=C5)=C5C(O)=O)=CC3=O)=C1.OC6=CC=C7C(OC(C(C=C8)=C7C(C=C(C(ON(C(CC9)=O)C9=O)=O)C=C%10)=C%10C(O)=O)=CC8=O)=C6
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Synonyms
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Nat Commun
Highly ordered macroporous hydrogen-bonded organic frameworks based on small biocompatible molecules. [Abstract]2025 Dec 8. PMID: 41360801 -
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Sci Adv
A rationally designed injury kidney targeting peptide library and its application in rescuing acute kidney injury. [Abstract]2025 May 2;11(18):eadt3943. PMID: 40315322 -
Solvent & Solubility
DMSO : 350 mg/mL (739.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Stafford JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter. J Biol Chem. 2001 Oct 26;276(43):39885-91. [Content Brief]
[2]. Powell RD, et al. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc Res Tech. 1998 Jul 1;42(1):2-12. [Content Brief]
[3]. Fulcher D, et al., Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory. Immunol Cell Biol. 1999 Dec;77(6):559-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1124 mL | 10.5621 mL | 21.1242 mL | 52.8106 mL |
| 5 mM | 0.4225 mL | 2.1124 mL | 4.2248 mL | 10.5621 mL | |
| 10 mM | 0.2112 mL | 1.0562 mL | 2.1124 mL | 5.2811 mL | |
| 15 mM | 0.1408 mL | 0.7041 mL | 1.4083 mL | 3.5207 mL | |
| 20 mM | 0.1056 mL | 0.5281 mL | 1.0562 mL | 2.6405 mL | |
| 25 mM | 0.0845 mL | 0.4225 mL | 0.8450 mL | 2.1124 mL | |
| 30 mM | 0.0704 mL | 0.3521 mL | 0.7041 mL | 1.7604 mL | |
| 40 mM | 0.0528 mL | 0.2641 mL | 0.5281 mL | 1.3203 mL | |
| 50 mM | 0.0422 mL | 0.2112 mL | 0.4225 mL | 1.0562 mL | |
| 60 mM | 0.0352 mL | 0.1760 mL | 0.3521 mL | 0.8802 mL | |
| 80 mM | 0.0264 mL | 0.1320 mL | 0.2641 mL | 0.6601 mL | |
| 100 mM | 0.0211 mL | 0.1056 mL | 0.2112 mL | 0.5281 mL |
5(6)-FAM SE Related Classifications
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