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Cat. No.: HY-163330

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8-Azakinetin riboside

8-Azakinetin riboside is a structural analog of kinetin riboside. Azakinetin riboside shows cytotoxic activity.

For research use only. We do not sell to patients.

CAS No.: 2347524-00-7
Formula: C₁₄H₁₆N₆O₅
Molecular Weight:348.31

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

8-Azakinetin riboside Chemical Structure

CAS No.: 2347524-00-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Protocols (2)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

8-Azakinetin riboside is a structural analog of kinetin riboside. Azakinetin riboside shows cytotoxic activity^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	11.5 μM Compound: 7; 8-azaKR	Cytotoxicity against human Caco-2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human Caco-2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 38280656]
Caco-2	IC₅₀	18.7 μM Compound: 7; 8-azaKR	Cytotoxicity against human Caco-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human Caco-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38280656]
Caco-2	IC₅₀	25 μM Compound: 7; 8-azaKR	Cytotoxicity against human Caco-2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human Caco-2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38280656]
HeLa	IC₅₀	>100 μM Compound: 7; 8-azaKR	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38280656]
HeLa	IC₅₀	14.5 μM Compound: 7; 8-azaKR	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38280656]
HeLa	IC₅₀	4.6 μM Compound: 7; 8-azaKR	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 38280656]
MCF7	IC₅₀	27 μM Compound: 7; 8-azaKR	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38280656]
MCF7	IC₅₀	3.8 μM Compound: 7; 8-azaKR	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 38280656]
MCF7	IC₅₀	4.3 μM Compound: 7; 8-azaKR	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38280656]
MIA PaCa-2	IC₅₀	>100 μM Compound: 7; 8-azaKR	Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38280656]
MIA PaCa-2	IC₅₀	1.1 μM Compound: 7; 8-azaKR	Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 38280656]
MIA PaCa-2	IC₅₀	2.8 μM Compound: 7; 8-azaKR	Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38280656]
MRC5	IC₅₀	>100 μM Compound: 7; 8-azaKR	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38280656]
MRC5	IC₅₀	11.7 μM Compound: 7; 8-azaKR	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38280656]
MRC5	IC₅₀	4.6 μM Compound: 7; 8-azaKR	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 38280656]
OVCAR-3	IC₅₀	>100 μM Compound: 7; 8-azaKR	Cytotoxicity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38280656]
OVCAR-3	IC₅₀	1.1 μM Compound: 7; 8-azaKR	Cytotoxicity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 38280656]
OVCAR-3	IC₅₀	76 μM Compound: 7; 8-azaKR	Cytotoxicity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38280656]

In Vitro

Azakinetin riboside (compound 7) (0-100 μM; 24, 48, 72 h) shows cytotoxic activity for HeLaWT, MCF7, Caco-2, OVCAR-3, MIAPaCa-2, MRC-5 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Cell Cytotoxicity Assay^[1]

View Protocol

Cell Line:

HeLaWT, MCF7, Caco-2, OVCAR-3, MIAPaCa-2, MRC-5 cells
Concentration:

0-100 µM
Incubation Time:

24, 48, 72 h
Result:

Showed cytotoxic activity for HeLaWT, MCF7, Caco-2, OVCAR-3, MIAPaCa-2, MRC-5 cells with IC₅₀s of >100, 27, 25, >100, >100, >100 µM at 24 h, 14.5, 4.3, 18.7, 76, 2.8, 11.7 at 48 h, 4.6, 3.8, 11.5, 1.1, 1.1, 4.6 µM at 72 h, respectively.

Chemical Information

CAS No. 2347524-00-7
Molecular Weight 348.31
Formula C₁₄H₁₆N₆O₅
SMILES

O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3NCC4=CC=CO4)=C3N=N2)O[C@@H]1CO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Totoń E, et al. Cytotoxic effects of kinetin riboside and its selected analogues on cancer cell lines. Bioorg Med Chem Lett. 2024 Mar 1;100:129628. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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