Cat. No.: HY-12802

AF38469

Name: AF38469
Brand: MedChemExpress
SKU: HY-12802
Price: 110.00 USD
Availability: InStock
Rating: 5 (10 reviews)

10 Cited Publications

Customer Review

Based on 10 publication(s) in Google Scholar

AF38469 is a selective, orally bioavailable Sortilin inhibitor with an IC₅₀ value of 330 nM.

For research use only. We do not sell to patients.

Purity: 99.28%
CAS No.: 1531634-31-7
Formula: C₁₅H₁₁F₃N₂O₃
Molecular Weight:324.25

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

AF38469 Chemical Structure

CAS No.: 1531634-31-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vivo
Chemical Information
Publications (10)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

AF38469 is a selective, orally bioavailable Sortilin inhibitor with an IC₅₀ value of 330 nM.

IC₅₀ & Target

IC50:330 nM (Sortilin)^[1]

All Neurotensin Receptor Isoforms :

NTR1
NTR2

In Vivo

AF38469 (1 mg/kg; i.v.) has a low volume of distribution (blood volume of 0.7 L/kg) and low clearance (4.8 L/h/kg), and a half-life (t1/2) of 1.2 h in rat^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1531634-31-7
Appearance Solid
Molecular Weight 324.25
Formula C₁₅H₁₁F₃N₂O₃
Color Off-white to light yellow
SMILES

O=C(NC1=CC=CC(C)=N1)C2=CC=C(C(F)(F)F)C=C2C(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (10)

All Cell Migration/Invasion AssayWBIn Vivo Efficacy StudyHistological Imaging/StainingIHC

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Journal Impact Factor

Cell Death Dis

Sortilin promotes glioblastoma invasion and mesenchymal transition through GSK-3β/β-catenin/twist pathway. [Abstract]

2019 Feb 27;10(3):208. PMID: 30814514

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (400 nM; 24 h) impaired the migratory capabilities U87 and A172 cell lines.

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (400 nM; 24 h) significantly attenuated the invasive capacities of U87 and A172 cell lines.

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (AF; 400 nM; 24 h) reduced the protein expression of N-cadherin, vimentin, and MMP-9 in U87 and A172 cell lines.

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (AF; 400 nM; 24 h) decreased the protein expression of p-GSK-3β, β-catenin, and Twist in both U87 and A172 cells.

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (AF38; 0, 100, 200, 400, 8000, 1600 nM; 0, 2, 4, 6, 8, 16, 24 h) decreased the protein expression of p-GSK-3β, β-catenin, and Twist in a dose- and time-dependent manner in U87 cells.

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (0.1 mg/kg; i.p.; daily for 21 days) showed the tumor volume of subcutaneous GBM was much smaller compared with the control group.

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (0.1 mg/kg; i.p.; daily for 21 days) decreased the activation of the GSK-3β/β-catenin/twist axis in tumor tissues and increased T-cadherin expression, while decreasing the levels of N-cadherin, vimentin, and MMP-9 in subcutaneous tumor tissues.

AF38469 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 27;10(3):208. [Abstract]

AF38469 (0.1 mg/kg; i.p.; daily for 21 days) showed that the tumor margin of the AF38469 group was clearer and smoother than that of the DMSO group, suggesting that AF38469 suppresses the invasive capacity of intracranial glioblastoma.
Cell Commun Signal

Presenilin1 inhibits glioblastoma cell invasiveness via promoting Sortilin cleavage. [Abstract]

2021 Nov 15;19(1):112. PMID: 34781973
J Neuroinflammation

Microglial progranulin differently regulates hypothalamic lysosomal function in lean and obese conditions via cleavage-dependent mechanisms. [Abstract]

2025 Mar 7;22(1):68. PMID: 40055725
Breast Cancer Res

Sortilin inhibition limits secretion-induced progranulin-dependent breast cancer progression and cancer stem cell expansion. [Abstract]

2018 Nov 20;20(1):137. PMID: 30454027

AF38469 purchased from MedChemExpress. Usage Cited in: Breast Cancer Res. 2018 Nov 20;20(1):137. [Abstract]

Immunohistochemical analyses of the GRN A- and AF38469-treated groups of MDA-MB 231 luc xenografts with skin is performed using Ki67 and haematoxylin staining.
Int J Mol Sci

Impact of Enniatin B and Beauvericin on Lysosomal Cathepsin B Secretion and Apoptosis Induction. [Abstract]

2023 Jan 19;24(3):2030. PMID: 36768354
Am J Pathol

The Membrane Protein Sortilin Can Be Targeted to Inhibit Pancreatic Cancer Cell Invasion. [Abstract]

2020 Sep;190(9):1931-1942. PMID: 32526166

AF38469 purchased from MedChemExpress. Usage Cited in: Am J Pathol. 2020 Sep;190(9):1931-1942. [Abstract]

Western blot detection of phosphorylation of FAK (Tyr925), FAK (Tyr397) and FAK (Tyr576/577) in the treatment with AF38469. The phosphorylation of residue Tyr925 of FAK is decreased in sortilin-inhibited compared to NT control.
Cancers (Basel)

Interleukin-6 Induces Stem Cell Propagation through Liaison with the Sortilin-Progranulin Axis in Breast Cancer. [Abstract]

2023 Dec 8;15(24):5757. PMID: 38136303
Cancers (Basel)

The Membrane Protein Sortilin Is a Potential Biomarker and Target for Glioblastoma. [Abstract]

2023 Apr 27;15(9):2514. PMID: 37173980
bioRxiv

2026 May 12.
bioRxiv

2025 March 11.

Solvent & Solubility

In Vitro:

DMSO : ≥ 43 mg/mL (132.61 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0840 mL

15.4202 mL

30.8404 mL

5 mM

0.6168 mL

3.0840 mL

6.1681 mL

10 mM

0.3084 mL

1.5420 mL

3.0840 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline

Solubility: 2.5 mg/mL (7.71 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Schroder TJ, et al. The identification of AF38469: an orally bioavailable inhibitor of the VPS10P family sorting receptor Sortilin. Bioorg Med Chem Lett. 2014 Jan 1;24(1):177-80. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0840 mL	15.4202 mL	30.8404 mL	77.1010 mL
	5 mM	0.6168 mL	3.0840 mL	6.1681 mL	15.4202 mL
	10 mM	0.3084 mL	1.5420 mL	3.0840 mL	7.7101 mL
	15 mM	0.2056 mL	1.0280 mL	2.0560 mL	5.1401 mL
	20 mM	0.1542 mL	0.7710 mL	1.5420 mL	3.8551 mL
	25 mM	0.1234 mL	0.6168 mL	1.2336 mL	3.0840 mL
	30 mM	0.1028 mL	0.5140 mL	1.0280 mL	2.5700 mL
	40 mM	0.0771 mL	0.3855 mL	0.7710 mL	1.9275 mL
	50 mM	0.0617 mL	0.3084 mL	0.6168 mL	1.5420 mL
	60 mM	0.0514 mL	0.2570 mL	0.5140 mL	1.2850 mL
	80 mM	0.0386 mL	0.1928 mL	0.3855 mL	0.9638 mL
	100 mM	0.0308 mL	0.1542 mL	0.3084 mL	0.7710 mL

Bioz

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

AF38469

SORT-PGRN interaction inhibitor 1

Sortilin antagonist 1

S63845

Ferrostatin-1

1-Aminobenzotriazole

Rapamycin

Neurotensin(8-13)

6PPD

Nile Red

SR 142948

LM11A-31 dihydrochloride

AF40431

Publications Citing Use of MedChemExpress (MCE) AF38469

All Neurotensin Receptor Isoforms

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

AF38469

SORT-PGRN interaction inhibitor 1

Sortilin antagonist 1

S63845

Ferrostatin-1

1-Aminobenzotriazole

Rapamycin

Neurotensin(8-13)

6PPD

Nile Red

SR 142948

LM11A-31 dihydrochloride

AF40431

Publications Citing Use of MedChemExpress (MCE) AF38469

All Neurotensin Receptor Isoforms

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

AF38469 Related Classifications

HY-12802 Related Classifications

Bioz