Sortilin antagonist 1
Based on 1 Customer Validation
Sortilin antagonist 1 is a sortilin antagonist with an IC50 of 0.02 μM. Sortilin antagonist 1 is used in research related to hearing loss.
For research use only. We do not sell to patients.
- Purity : 99.60%
- CAS No.: 2691846-93-0
- Formula: C20H24N2O4
- Molecular Weight:356.42
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-12802
AF38469Sortilin Inhibitor -
HY-107664
SR 142948NT Antagonist -
HY-125880
SBI-553NTR1 Allosteric Modulator -
![[Lys8, Lys9]-Neurotensin (8-13)](https://file.medchemexpress.com/product_pic/hy-p2544.gif)
HY-P2544
[Lys8, Lys9]-Neurotensin (8-13) -
HY-137322
THX-Bp75NTR Antagonist -
HY-115213
SORT-PGRN interaction inhibitor 1Sortilin-Progranulin Interaction Inhibitor -
HY-124673
AF40431Sortilin Ligand -
HY-12436
NTRC-824NTS2 Antagonist -
HY-14551B
(S)-OsanetantNK3 Receptor Antagonist -
HY-148650A
(R)-Sortilin antagonist 1Sortilin Antagonist -
HY-107664R
SR 142948 (Standard)Reference Standard
Biological Activity
Description
In Vitro
Sortilin antagonist 1 (compound 44) inhibits the interaction between SORT and biotinylated 18-mer PGRN peptide in the PGRN peptide/SORT HTRF assay, with an IC50 of 0.02 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2691846-93-0
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Appearance Solid
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Molecular Weight 356.42
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Formula C20H24N2O4
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Color White to off-white
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SMILES
CC(C)(C)CC[C@@H](C(O)=O)NC(C(C=N1)=CC=C1OC2=CC=CC=C2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (280.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8057 mL | 14.0284 mL | 28.0568 mL | 70.1420 mL |
| 5 mM | 0.5611 mL | 2.8057 mL | 5.6114 mL | 14.0284 mL | |
| 10 mM | 0.2806 mL | 1.4028 mL | 2.8057 mL | 7.0142 mL | |
| 15 mM | 0.1870 mL | 0.9352 mL | 1.8705 mL | 4.6761 mL | |
| 20 mM | 0.1403 mL | 0.7014 mL | 1.4028 mL | 3.5071 mL | |
| 25 mM | 0.1122 mL | 0.5611 mL | 1.1223 mL | 2.8057 mL | |
| 30 mM | 0.0935 mL | 0.4676 mL | 0.9352 mL | 2.3381 mL | |
| 40 mM | 0.0701 mL | 0.3507 mL | 0.7014 mL | 1.7535 mL | |
| 50 mM | 0.0561 mL | 0.2806 mL | 0.5611 mL | 1.4028 mL | |
| 60 mM | 0.0468 mL | 0.2338 mL | 0.4676 mL | 1.1690 mL | |
| 80 mM | 0.0351 mL | 0.1754 mL | 0.3507 mL | 0.8768 mL | |
| 100 mM | 0.0281 mL | 0.1403 mL | 0.2806 mL | 0.7014 mL |
Sortilin antagonist 1 Related Classifications
HY-148650 Related Classifications
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