Diacetazotol (Synonyms: Diacetotoluide)

Cat. No.: HY-B2187 Purity: ≥90.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Diacetazotol inhibits dioxin-induced ethoxyresorufin-O-deethylase (EROD) activity with IC₅₀ of 75±4 nM. Diacetazotol extracts from patent US20070032458, compound 3.

For research use only. We do not sell to patients.

Diacetazotol Chemical Structure

CAS No. : 83-63-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 41	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 41	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Description

Diacetazotol inhibits dioxin-induced ethoxyresorufin-O-deethylase (EROD) activity with IC₅₀ of 75±4 nM. Diacetazotol extracts from patent US20070032458, compound 3.

IC₅₀ & Target

IC50: 75±4 nM (Dioxin-induced EROD activity)^[1]

In Vitro

Diacetazotol has activity for inhibiting dioxin toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

309.36

Formula

C₁₈H₁₉N₃O₂

CAS No.

83-63-6

Appearance

Solid

Color

Pink to red

SMILES

CC(N(C(C)=O)C1=CC=C(/N=N/C2=CC=CC=C2C)C=C1C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (323.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2325 mL	16.1624 mL	32.3248 mL
5 mM	0.6465 mL	3.2325 mL	6.4650 mL
10 mM	0.3232 mL	1.6162 mL	3.2325 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.08 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥90.0%

Select Batch:

Data Sheet (270 KB) SDS (0 KB)

COA (247 KB) HNMR (285 KB)

Handling Instructions (2659 KB)

References

[1]. Pann Suh, et al. Compound having activity for inhibiting dioxin toxicity, pharmaceutical composition comprising the compound and method for treating disease resulting from dioxin toxicity using the compound .US 20070032458 A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2325 mL	16.1624 mL	32.3248 mL	80.8120 mL
	5 mM	0.6465 mL	3.2325 mL	6.4650 mL	16.1624 mL
	10 mM	0.3232 mL	1.6162 mL	3.2325 mL	8.0812 mL
	15 mM	0.2155 mL	1.0775 mL	2.1550 mL	5.3875 mL
	20 mM	0.1616 mL	0.8081 mL	1.6162 mL	4.0406 mL
	25 mM	0.1293 mL	0.6465 mL	1.2930 mL	3.2325 mL
	30 mM	0.1077 mL	0.5387 mL	1.0775 mL	2.6937 mL
	40 mM	0.0808 mL	0.4041 mL	0.8081 mL	2.0203 mL
	50 mM	0.0646 mL	0.3232 mL	0.6465 mL	1.6162 mL
	60 mM	0.0539 mL	0.2694 mL	0.5387 mL	1.3469 mL
	80 mM	0.0404 mL	0.2020 mL	0.4041 mL	1.0102 mL
	100 mM	0.0323 mL	0.1616 mL	0.3232 mL	0.8081 mL

Diacetazotol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diacetazotol83-63-6DiacetotoluideOthersInhibitorinhibitorinhibit

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