E-6123
E-6123 is a platelet-activating factor (PAF) receptor antagonist.
For research use only. We do not sell to patients.
- CAS No.: 131614-02-3
- Formula: C23H22ClN5OS
- Molecular Weight:451.97
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PAF[1]
E-6123 (E6123) partially inhibits the Thapsigargin-induced increase in the levels of interleukin-6 mRNA and interleukin-6 protein. To clarify the role of the concurrently produced cell-associated PAF in the thapsigargin-induced increase in the interleukin-6 mRNA level and interleukin-6 production, the effects of the PAF receptor antagonist E-6123 are examined. E-6123 at 1 and 10 μM partially inhibits the Thapsigargin (46.1 nM)-induced increase in the interleukin-6 mRNA level at 4 h and interleukin-6 production at 8 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 131614-02-3
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Molecular Weight 451.97
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Formula C23H22ClN5OS
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SMILES
O=C(C1CC1)N2CC3=C(CC2)C(C(C4=CC=CC=C4Cl)=N[C@H]5C)=C(S3)N6C5=NN=C6C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
The viability of the cells is examined in each set of experiments by a procedure using MTT, which is based on the ability of mitochondrial succinate dehydrogenase to cleave MTT to the blue compound formazan. After the peritoneal cells are incubated for the periods indicated in 10 mL of medium containing the various drugs (e.g., E-6123), 1 mL of MTT solution in phosphate-buffered saline (5 mg/mL, pH 7.4) is added to each dish, and the cells are further incubated for 4 h at 37 °C. Next, 2.5 mL of 0.04 N HCl solution in isopropanol is added, and the cells are sonicated at 10% maximum power for 3 s. The resultant colored product is read on a Microplate Reader at 570 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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