PKG Substrate
Based on 1 Customer Validation
PKG Substrate is a selective substrate for cGMP-dependent protein kinase (PKG).
For research use only. We do not sell to patients.
- Purity : 98.97%
- CAS No.: 81187-14-6
- Formula: C35H67N17O11
- Molecular Weight:902.01
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
PKG[1]
In Vitro
Incorporation of [33P]ATP into the synthetic peptide PKG substrate RKRSRAE is measured. N6-benzyl-ATP inhibits kinase activity of PKG Iα gatekeeper mutants but not WT. The serotonin transporter (SERT) is responsible for reuptake of serotonin (5-hydroxytryptamine) after its exocytotic release from neurons. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and PKG[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 81187-14-6
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Appearance Solid
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Molecular Weight 902.01
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Formula C35H67N17O11
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Color White to off-white
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Sequence
Arg-Lys-Arg-Ser-Arg-Ala-Glu
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Sequence Shortening
RKRSRAE
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (110.86 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (110.86 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1086 mL | 5.5432 mL | 11.0864 mL | 27.7159 mL |
| 5 mM | 0.2217 mL | 1.1086 mL | 2.2173 mL | 5.5432 mL | |
| 10 mM | 0.1109 mL | 0.5543 mL | 1.1086 mL | 2.7716 mL | |
| 15 mM | 0.0739 mL | 0.3695 mL | 0.7391 mL | 1.8477 mL | |
| 20 mM | 0.0554 mL | 0.2772 mL | 0.5543 mL | 1.3858 mL | |
| 25 mM | 0.0443 mL | 0.2217 mL | 0.4435 mL | 1.1086 mL | |
| 30 mM | 0.0370 mL | 0.1848 mL | 0.3695 mL | 0.9239 mL | |
| 40 mM | 0.0277 mL | 0.1386 mL | 0.2772 mL | 0.6929 mL | |
| 50 mM | 0.0222 mL | 0.1109 mL | 0.2217 mL | 0.5543 mL | |
| 60 mM | 0.0185 mL | 0.0924 mL | 0.1848 mL | 0.4619 mL | |
| 80 mM | 0.0139 mL | 0.0693 mL | 0.1386 mL | 0.3464 mL | |
| 100 mM | 0.0111 mL | 0.0554 mL | 0.1109 mL | 0.2772 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
PKG Substrate Related Classifications
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