1. Cell Cycle/DNA Damage
  2. CDK
  3. Purvalanol B

Purvalanol B (Synonyms: NG 95)

Cat. No.: HY-18299 Purity: >97.0% ee.: 99.59%
Handling Instructions

Purvalanol B(NG-95) is a cyclin-dependent kinase inhibitor with IC50 values of 6, 6, 9, > 10,000, and 6 nM for cdc2/cyclin B, cdk2/cyclin A, cdk2/cyclin E, cdk4/cyclin D1 and cdk5-p35 respectively.

For research use only. We do not sell to patients.

Purvalanol B Chemical Structure

Purvalanol B Chemical Structure

CAS No. : 212844-54-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 82 In-stock
Estimated Time of Arrival: December 31
5 mg USD 74 In-stock
Estimated Time of Arrival: December 31
10 mg USD 99 In-stock
Estimated Time of Arrival: December 31
50 mg USD 250 In-stock
Estimated Time of Arrival: December 31
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Description

Purvalanol B(NG-95) is a cyclin-dependent kinase inhibitor with IC50 values of 6, 6, 9, > 10,000, and 6 nM for cdc2/cyclin B, cdk2/cyclin A, cdk2/cyclin E, cdk4/cyclin D1 and cdk5-p35 respectively. IC50 Value: 6 nM(cdc2/cyclin B); 6 nM(cdk2/cyclin A); 9 nM(cdk2/cyclin E); 6 nM(cdk5-p35)[1] Target: cdc2/cyclin B; cdk2/cyclin E; cdk5-p35 in vitro: In vitro inhibitory activity against Cyclin-dependent kinase 1-cyclin B complex from starfish oocytes is 6 nM (IC50) [1]. In addition to CDK1, p42/p44 MAPK were found to be two major purvalanol-interacting proteins in five different mammalian cell lines (CCL39, PC12, HBL100, MCF-7 and Jurkat cells), suggesting the generality of the purvalanol/p42/p44 MAPK interaction. When cells were treated with purvalanol, p42/p44 MAPK and CDK1 activities were inhibited in a dose-dependent manner. Furthermore, purvalanol inhibited the nuclear accumulation of p42/p44 MAPK, an event dependent on the catalytic activity of these kinases [2]. in vivo:

IC50 & Target[1]

cdc2/cyclin B

6 nM (IC50)

cyclin dependent kinase 1-cyclinB

6 nM (IC50)

cdk2/cyclin A

6 nM (IC50)

CDK2/cyclinE

9 nM (IC50)

CDK5/p35

6 nM (IC50)

Cdk4/cyclin D1

10000 nM (IC50)

TrkA

51 nM (IC50)

TrkB

11 nM (IC50)

Molecular Weight

432.90

Formula

C₂₀H₂₅ClN₆O₃

CAS No.

212844-54-7

SMILES

O=C(O)C1=CC=C(NC2=C3N=CN(C(C)C)C3=NC(N[[email protected]@H](CO)C(C)C)=N2)C=C1Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 40 mg/mL (92.40 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3100 mL 11.5500 mL 23.1000 mL
5 mM 0.4620 mL 2.3100 mL 4.6200 mL
10 mM 0.2310 mL 1.1550 mL 2.3100 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.78 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.78 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: >97.0% ee.: 99.59%

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Keywords:

Purvalanol BNG 95NG95NG-95CDKCyclin dependent kinaseInhibitorinhibitorinhibit

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Purvalanol B
Cat. No.:
HY-18299
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