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RS-246204 

Cat. No.: HY-112484
Handling Instructions

RS-246204 is a R-spondin-1 substitute compound that is able initiate small intestinal organoids without the use of the R-spondin-1 protein.

For research use only. We do not sell to patients.

RS-246204 Chemical Structure

RS-246204 Chemical Structure

CAS No. : 878451-87-7

Size Price Stock
5 mg USD 350 Get quote
10 mg USD 550 Get quote
25 mg USD 1100 Get quote
50 mg USD 1750 Get quote
100 mg USD 2750 Get quote

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

RS-246204 is a R-spondin-1 substitute compound that is able initiate small intestinal organoids without the use of the R-spondin-1 protein.

In Vitro

R-spondin-1 protein is added at a high concentration for the initiation and maintenance of the organoids. RS-246204 is a R-spondin-1 substitute that could promote the initial formation and growth of enteroids in the medium without R-spondin-1. The enteroids grown with RS-246204 had a similar differentiation capacity as well as self-renewal capacity as the enteroids grown with R-spondin-1. Furthermore, the RS-246204-derived enteroids could successfully produce the forskolin induced swelling and the organoid based epithelial to mesenchymal transition model[1].

In Vivo

RS-246204 significantly increased BrdU+ cells and body weight in the DSS induced colitis model. These results suggest that RS-246204 activates the R-spondin dependent pathway in Lgr5 stem cells, thereby enhancing proliferation or the anti-apoptotic effect, promoting regeneration of the intestinal epithelium and reducing the disease severity[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9466 mL 14.7332 mL 29.4664 mL
5 mM 0.5893 mL 2.9466 mL 5.8933 mL
10 mM 0.2947 mL 1.4733 mL 2.9466 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[1]

Mice[1]

Seven-week-old C57Bl/6 mice are given a 2.5% dextran sulfate sodium treatment in drinking water for 5 days. RS-246204 (10 mg/kg in DMSO) is administered intraperitoneally at the end of the DSS exposure. The RS-246204 treatment is repeated every 24 h for 5 days. Daily assessments of disease activity are performed including measuring body weight and evaluating stool consistency. The mice are sacrificed the next day after the last injection of RS-246204. BrdU (50 mg/kg in saline) is administered intraperitoneally 24 hours before sacrifice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

339.37

Formula

C₁₆H₁₃N₅O₂S

CAS No.

878451-87-7

SMILES

O=C(N1)N(C)C2=C(N(C)C(SC3=NC4=CC=CC=C4C=C3)=N2)C1=O

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
RS-246204
Cat. No.:
HY-112484
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RS-246204

Cat. No.: HY-112484