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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1883B
    Cy7.5-COOH bromide
    Cy7.5-COOH bromide, a cyanine dye, is a highly fluorescent compound (Abs/Em = 781/808 nm). Cy7.5-COOH bromide can be used as a fluorescent probe in a wide-range of applications such as DNA sequencing, flow cytometry and in vivo imaging.
    Cy7.5-COOH bromide
  • HY-W123012
    APN-Azide
    		99.1%
    APN-Azide (3-(4-Azidophenyl)propiolonitrile) is a codon-active compound that can achieve specific labeling of target molecules in biological systems through its unique chemical structure. APN-Azide can be used for bioimaging and the development of molecular probes to study biological processes within cells.
    APN-Azide
  • HY-165426
    HB-230
    HB-230 is a red fluorescent transglutaminase 2 (TG2) probe. HB-230 complexes with TG2 and α2-macroglobulin via the LRP1 pathway for efficient endocytosis. The excitation and emission wavelengths of HB-230 are 649 and 665 nm, respectively.
    HB-230
  • HY-15563
    HOE 33187
    		99.36%
    HOE 33187 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    HOE 33187
  • HY-D2441
    TAT-PEG-Cy3
    TAT-PEG-Cy3 is a fluorescent labeling reagent that combines Cy3 fluorescent dye, Cell membrane penetrating peptide (TAT) and polyethylene glycol (PEG). The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has an emission maximum around 562-570 nm. TAT-PEG-Cy3 can be used for cell targeted delivery and biological imaging.
    TAT-PEG-Cy3
  • HY-W415108
    10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate
    		99.41%
    10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate is an acridinium ester that produces fluorescent 10-methyl-9-acridone upon oxidation with hydrogen peroxide, persulfate, and other oxidants under alkaline conditions. 10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate can be used in chemiluminescent assays, enzyme, antigen, antibody, and hormone immunoassays, and for the detection of oxidants in environmental, biological, and pharmaceutical samples.
    10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate
  • HY-D0429
    C.I. Pigment red 5
    C.I. Pigment red 5 is the most important colorant used to add color or change the color of something and is chemically essentially unaffected by the carrier or medium into which it is incorporated. It can be widely used in textile, medicine, food, cosmetics, plastics, paint, ink, photography and paper industries.
    C.I. Pigment red 5
  • HY-15626
    ortho-iodoHoechst 33258
    		98.01%
    ortho-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    ortho-iodoHoechst 33258
  • HY-P3123A
    Dnp-RPLALWRS TFA
    		99.04%
    Dnp-RPLALWRS TFA is a fluorescent peptide substrate designed for human matrilysin (MMP-7). After enzymatic cleavage of Dnp-RPLALWRS TFA at the alanine-leucine bond, the release of the Dnp group alleviates fluorescence quenching, thereby enabling real-time quantitative analysis of MMP-7 activity by increasing tryptophan emission. Dnp-RPLALWRS TFA provides a sensitive and efficient detection method for kinetic studies and inhibitor screenin.
    Dnp-RPLALWRS TFA
  • HY-160277
    DSPE-PEG2000-Fluor 594
    DSPE-PEG2000-Fluor 594 is a PEGylated lipid with a DSPE group and a Fluor 594 fluorophore. DSPE is a phospholipid. Fluor 594 is a red water-soluble dye.
    DSPE-PEG2000-Fluor 594
  • HY-172164B
    FITC-lysine-dextran (MW 70kDa)
    FITC-lysine-dextran, 70kDa is a dextran with lysine coupling with a fluorescent group FITC (HY-66019). Lysine-dextran exhibits higher Glu-plasminogen binding ability than dextran alone.
    FITC-lysine-dextran (MW 70kDa)
  • HY-D0492
    C.I. Basic blue 41
    C.I. Basic blue 41 is an alkaline dye that can be removed from aqueous solutions in intermittent adsorption systems using tree ferns as biosorbents.
    C.I. Basic blue 41
  • HY-176513
    Cy-OH-LP
    		99.0%
    Cy-OH-LP is a turn-on fluorescent and photoacoustic probe that can detect lipid-associated hydroxyl radicals. Cy-OH-LP can be oxidized to Cy7 in the presence of hydroxyl radicals, which displays weak fluorescent and photoacoustic signals in water-soluble environments and strong fluorescent and photoacoustic signals in lipid-rich environments due to its lipid solubility. Cy-OH-LP can be used for in vivo photoacoustic imaging of hydroxyl radicals.
    Cy-OH-LP
  • HY-D2267
    JF646-Hoechst
    JF646-Hoechst is a fluorescent red DNA probe that is an ideal substitute for large oligonucleotide-coupled antibodies used in PAINT experiments, especially for bacterial studies. JF646-Hoechst excitation/emission maximum =655/670 nm.
    JF646-Hoechst
  • HY-D2498
    Cy7 maleimide
    Cy7 maleimide is a fluorescent dye containing a Maleimide (HY-W007324) functional group. Maleimide can be used to label thiol groups on antibodies, proteins and peptides.
    Cy7 maleimide
  • HY-D1376
    Sulfo-Cyanine5.5 amine potassium
    Sulfo-Cyanine5.5 amine potassium is a water-soluble cyanine dye suitable for far-red/near-infrared applications such as in vivo imaging. The dye has four sulfonate groups, making it highly hydrophilic and water-soluble. Like other cyanines, sulfo-Cyanine5.5 has an excellent extinction coefficient, making it a bright fluorescent marker in the far-red region. This is an amine-containing fluorescent dye. The amine group is separated from the fluorophore by a relatively long linker that facilitates conjugation. Aliphatic primary amine groups can be coupled with various electrophiles (activated esters, epoxides, etc.) and can also be used for enzymatic transamination labeling.
    Sulfo-Cyanine5.5 amine potassium
  • HY-D2709
    Cy3 Dextran (MW 20000)
    Cy3 Dextran (MW 20000) is a fluorescent labeling reagent that combines Cy3 (HY-D0822) fluorescent dye and Dextran (HY-112624). The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Dextran has an inhibitory effect on thrombocyte aggregation and coagulation factors and is used as a plasma volume expander (Ex/Em = 550/570 nm).
    Cy3 Dextran (MW 20000)
  • HY-D0739
    Disperse yellow 241
    		98.04%
    Disperse yellow 241 is a yellow dye commonly used for fiber dyeing.
    Disperse yellow 241
  • HY-W329161
    Calcein sodium salt
    Calcein sodium is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems.
    Calcein sodium salt
  • HY-78315
    2',3',4'-Trimethoxyacetophenone
    2',3',4'-Trimethoxyacetophenone is an organic compound with a benzene ring and an acetyl group. 2',3',4'-Trimethoxyacetophenone can be used as a raw material for synthesizing dyes, fragrances, and certain pesticides.
    2',3',4'-Trimethoxyacetophenone
Cat. No. Product Name / Synonyms Application Reactivity