1. Neuronal Signaling
  2. Amyloid-β

β-amyloid 12-28 (Synonyms: Amyloid β-Protein (12-28))

Cat. No.: HY-P1051
Handling Instructions

β-amyloid (12-28) is a 17-aa peptide fragment, which can produce amyloid aggregates, used in the research of Alzheimer’s disease.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

β-amyloid 12-28 Chemical Structure

β-amyloid 12-28 Chemical Structure

CAS No. : 107015-83-8

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

β-amyloid (12-28) is a 17-aa peptide fragment, which can produce amyloid aggregates, used in the research of Alzheimer’s disease.

IC50 & Target

Amyloid-β[1]

In Vitro

β-amyloid (12-28) preared freshly in solution has no cytotoxicity, but aged β12-28 peptide (0.001-40 μM) reduces the viability of PC12 cells in a concentration dependent manner[1].

References
Cell Assay
[1]

PC12 pheochromocytoma cell lines are routinely cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum,5% horse serum, 1% glutamine and 1% penicillin/streptomycin. Exponentially growing cellsare plated at 30000 cells per well per 100 µL of fresh medium in 96-well tissue cultures plates. Toxicity assays are carried out by measurementof MTT reduction. Two days after plating PC12 pheochromocytoma cell lines are treated with different concentrations of aged β-amyloid (12-28). After 22 h, MTT (5 mg/mL) wasadded and incubation is continued for a further2 h. Cells are lysed and after 24 h at 37°C,colorimetric determination of MTT reduction is made at 550 nm. Positive controls consist of cells in culture medium. Assay values obtained with these controls are taken as 100% viability[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1955.18

Formula

C₈₉H₁₃₅N₂₅O₂₅

CAS No.

107015-83-8

Sequence

Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys

Sequence Shortening

VHHQKLVFFAEDVGSNK

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
β-amyloid 12-28
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HY-P1051
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β-amyloid 12-28

Cat. No.: HY-P1051