Acapatamab
Based on 1 Customer Validation
Acapatamab (AMG-160) is an anti-CD3E/FOLH1 monoclonal antibody.
For research use only. We do not sell to patients.
- Purity : 98.35%
- CAS No.: 2314491-93-3
- Molecular Weight:105.72 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Isotype
(scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
CD3E & FOLH1
In Vitro
Acapatamab (AMG-160; 0-10000 pM; 48 h) induces T-cell activation and increases in CD25, CD69, 4-1BB, and PD-1 expression on T cells[1].
Acapatamab (0-10000 pM; 48 h; T-cell) induces dose-dependent secretion of cytokines, including IL2, IL4, IL6, IL10, IFNγ, and TNFα[1].
Acapatamab (0-10000 pM; 48 h) induces potent lysis of prostate-specific membrane antigen (PSMA)-positive prostate cancer cell lines in cocultures with PBMC, with EC50 values of 6-42 pM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NOD/SCID mice with 22Rv-1 CRPC xenografts[1]
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Dosage:0.02, 0.2, and 2 mg/kg
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Administration:Intravenous injection (days 16, 23, and 31)
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Result:Had antitumor activity against established CRPC tumors.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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(scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow cytometric analysis of 1×106 Jurkat cells labelling CD3E (red) with Acapatamab (HY-P99418). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control.
Chemical Information
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CAS No. 2314491-93-3
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Appearance Liquid
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Molecular Weight 105.72 kDa
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Color Colorless to light yellow
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SMILES
[Acapatamab]
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Synonyms
AMG-160
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (257 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Acapatamab
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)