Acapatamab
Based on 1 Customer Validation
Acapatamab (AMG-160) is an anti-CD3E/FOLH1 monoclonal antibody.
For research use only. We do not sell to patients.
- Purity: 98.35%
- CAS No.: 2314491-93-3
- Molecular Weight:105.72 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
(scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc
Human
CD3E & FOLH1
Acapatamab (AMG-160; 0-10000 pM; 48 h) induces T-cell activation and increases in CD25, CD69, 4-1BB, and PD-1 expression on T cells[1].
Acapatamab (0-10000 pM; 48 h; T-cell) induces dose-dependent secretion of cytokines, including IL2, IL4, IL6, IL10, IFNγ, and TNFα[1].
Acapatamab (0-10000 pM; 48 h) induces potent lysis of prostate-specific membrane antigen (PSMA)-positive prostate cancer cell lines in cocultures with PBMC, with EC50 values of 6-42 pM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NOD/SCID mice with 22Rv-1 CRPC xenografts[1]
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Dosage:0.02, 0.2, and 2 mg/kg
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Administration:Intravenous injection (days 16, 23, and 31)
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Result:Had antitumor activity against established CRPC tumors.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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(scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc
ELISA, FACS, Functional assay
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Flow cytometric analysis of 1×106 Jurkat cells labelling CD3E (red) with Acapatamab (HY-P99418). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control.
Chemical Information
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CAS No. 2314491-93-3
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Appearance Liquid
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Molecular Weight 105.72 kDa
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Color Colorless to light yellow
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SMILES
[Acapatamab]
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Synonyms
AMG-160
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (257 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Acapatamab
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)