AF 555 azide 

AF 555 azide is a fluorescent dye and is the azide of AF 555. It contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 555/565 nm)^[1][2].

AF555-azide can be used for copper-catalyzed azide-alkynylene cycloaddition reactions (CuAAc): AF555 azide can undergo copper-catalyzed azide-alkynylene cycloaddition reactions with molecules containing alkynyl groups to form stable 1,2,3-triazole rings.
AF555-azide can also undergo ring-strain-driven alkynylene-azide cycloaddition reactions with molecules containing DBCO or BCN groups without the need for a copper catalyst.
AF555-azide can be used for surface modification of nanoparticles, integrating fluorescent signals onto the nanoparticle surface through click chemistry reactions for fluorescent labeling and functionalization of materials.
AF555-azide can be used to modify polymers, achieving fluorescent labeling through reaction with alkynyl groups on the polymer chain.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1043.38

C₃₈H₄₉K₃N₆O₁₃S₄

Solid

Brown to black

CC(C)(/C1=C\C=C\C(C2(C)CCCCS(=O)(O[K])=O)=[N+](C3=C2C=C(C=C3)S(=O)(O[K])=O)CCCS(=O)([O-])=O)C4=C(C=CC(S(=O)(O[K])=O)=C4)N1CCCCCC(NCCCN=[N+]=[N-])=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Tsai M Y, et al. Vapor‐Based Multicomponent Coatings for Antifouling and Biofunctional Synergic Modifications[J]. Advanced Functional Materials, 2014, 24(16): 2281-2287.

  [2]. Gebhardt C, Lehmann M, Reif M M, et al. Molecular and spectroscopic characterization of green and red cyanine fluorophores from the Alexa Fluor and AF series[J]. ChemPhysChem, 2021, 22(15): 1566-1583.