2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Aldehyde Dehydrogenase (ALDH)
  4. AlDeSense AM

AlDeSense AM is a cell-permeable ALDH1A1-selective fluorescent reporter. AlDeSense AM is oxidized by ALDH1A1, which eliminates photoinduced electron transfer quenching and enhances the fluorescent signal. AlDeSense AM can be used to detect cells with cancer stem cell properties, as well as to monitor the plasticity of cancer stem cells in cell culture systems and animal models. AlDeSense AM is applicable to the study of cancers associated with cancer stem cells, including chronic myeloid leukemia, melanoma, and breast cancer.

For research use only. We do not sell to patients.

AlDeSense AM

AlDeSense AM Chemical Structure

CAS No. : 2243148-62-9

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AlDeSense AM Related Antibodies

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Description

AlDeSense AM is a cell-permeable ALDH1A1-selective fluorescent reporter. AlDeSense AM is oxidized by ALDH1A1, which eliminates photoinduced electron transfer quenching and enhances the fluorescent signal. AlDeSense AM can be used to detect cells with cancer stem cell properties, as well as to monitor the plasticity of cancer stem cells in cell culture systems and animal models. AlDeSense AM is applicable to the study of cancers associated with cancer stem cells, including chronic myeloid leukemia, melanoma, and breast cancer[1].

In Vitro

AlDeSense AM (1.5-2 μM; 30 min) selectively labels human chronic myeloid leukemia K562 cells with active ALDH1A1, which correspond to the CD34+/CD38−/CD133+ leukemia stem cell population[1].
AlDeSense AM (2 μM; 1 h) detects elevated ALDH1A1 activity in MDA-MB-231 breast cancer mammospheres (cancer stem cell-enriched), and the fluorescent signal decreases as the mammospheres differentiate over 36 h[1].
The fluorescence signal of AlDeSense AM (2 μM; 1 h) in CSC-enriched B16F0 e-CSC is 11.3-fold higher than that in non-CSC B16F0 cells, confirming elevated ALDH1A1 activity in e-CSC[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AlDeSense AM Related Antibodies

Immunofluorescence[1]

Cell Line: K562 human chronic myeloid leukemia cells
Concentration: 1.5 μM (flow cytometry staining); 2 μM (confocal imaging staining)
Incubation Time: 30 min at room temperature (confocal imaging staining)
Result: Identified a population of brightly fluorescent cells corresponding to the CD34+/CD38-/CD133+ leukemic stem cell profile.
Showed a small population of highly fluorescent cells via confocal imaging.
Completely abolished the fluorescence signal after ALDH1A1 siRNA knockdown.

Immunofluorescence[1]

Cell Line: MDA-MB-231 breast cancer mammospheres (CSC-enriched) and differentiating mammospheres
Concentration: 2 μM
Incubation Time: 1 h at room temperature (at 1, 6, 12, 24, 36 h time points during differentiation)
Result: Exhibited 3-fold brighter fluorescence in freshly isolated mammospheres compared to control-stained mammospheres.
Gradually decreased fluorescence signal over 36 h of differentiation.

Immunofluorescence[1]

Cell Line: B16F0 murine melanoma e-CSCs (CSC-enriched via spiral-patterned hydrogel culture) and non-CSCs (standard culture)
Concentration: 2 μM
Incubation Time: 1 h at room temperature
Result: Showed 11.3-fold brighter fluorescence in e-CSCs than in non-CSCs.
Showed 9.0-fold brighter fluorescence in e-CSCs than in e-CSCs stained with control reagent.
Colocalized with the CD271 melanoma stem cell marker in e-CSCs.
In Vivo

AlDeSense AM (15 μM; lung perfusion; single dose) selectively detects elevated ALDH1A1 activity in e-CSC-derived lung metastases in C57BL/6J mice, producing a significantly higher fluorescence signal than in non-CSC-derived metastases[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (female, 6 to 8 weeks old)[1]
Dosage: 15 μM
Administration: lung perfusion; single dose
Result: Produced a mean fluorescence signal of ~1×1011 a.u.
in lungs with e-CSC metastases.
Showed a significantly higher mean fluorescence signal (~1×1011 a.u.) than the ~7×1010 a.u.
measured in lungs with non-CSC metastases.
Demonstrated significantly higher fluorescence in e-CSC metastases than e-CSC metastases treated with Ctrl-AlDeSense AM.
Molecular Weight

438.38

Formula

C24H16F2O6
CAS No.
SMILES

CC(OCOC1=C(C=C2C(OC3=CC(C(F)=CC3=C2C4=CC=C(C=C4C)C=O)=O)=C1)F)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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AlDeSense AM Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AlDeSense AM2243148-62-9Fluorescent DyeAldehyde Dehydrogenase (ALDH)melanomabreast cancerCD34+/CD38-/CD133+ leukemic stem cell populationC57BL/6J micechronic myeloid leukemiaMDA-MB-231 breast cancer mammospherescancer stem cellsB16F0 e-CSCsK562 human chronic myeloid leukemia cellsALDH1A1Inhibitorinhibitorinhibit

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