AlkB (D135S/L118V), Nuclease-free

Nuclease-free AlkB (D135S/L118V) demethylates the m²₂G substrate, which is a poor substrate for wild-type AlkB^[1].
When incubated as a component of a three-enzyme mixture, AlkB (D135S/L118V), Nuclease-free (50-250 pmol; 30 min at 25°C), exhibits in vitro demethylase activity toward m¹A- and m¹G-modified RNA oligonucleotides^[2].
When mixed with 500 pmol each of AlkB and AlkB D135S, AlkB (D135S/L118V), Nuclease-free (500 pmol; 30 min at 25°C), effectively removes m¹A, m¹G, m³C and m²²G modifications from isolated zebrafish tRNA, and improves reverse transcription coverage in tRAM-seq analysis^[2].
Nuclease-free AlkB (D135S/L118V) efficiently and selectively removes one methyl group from m₂²G in synthetic 9-mer RNA oligonucleotides to generate m²G, and shows no activity against m²G^[3].
AlkB (D135S/L118V), Nuclease-free exhibits only limited demethylation activity towards m¹G in synthetic 9mer RNA oligonucleotides, showing substrate selectivity distinctly different from that of the AlkB D135S mutant^[3].
Nuclease-free AlkB (D135S/L118V) enhances the demethylation of m₂²G26 in tRNAs of HEK293T cells, reduces modification index values and improves tRNA sequencing efficiency, with the most significant effect observed on mitochondrial tRNAᴵˡᵉ^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.