AMP-PNP lithium hydrate
Based on 1 publication(s) in Google Scholar
AMP-PNP (Adenylyl imidodiphosphate) lithium hydrate is a non-hydrolyzable ATP analog. AMP-PNP lithium hydrate binds to ATP binding sites competely but is not hydrolyzed by enzymes, providing stable experimental conditions for studying ATP-dependent processes. AMP-PNP lithium hydrate can also be used to study enzyme activity, kinase regulation, DNA/RNA metabolism, ion channel function, and protein complex assembly.
For research use only. We do not sell to patients.
- Purity : 99.98%
- Formula: C10H17N6O12P3·xLi·yH2O
- Molecular Weight:506.20 (free acid)
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) AMP-PNP lithium hydrate
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Biological Activity
Description
In Vitro
AMP-PNP lithium hydrate (100 nM-3.3 mM) competitively elutes BtuCD(EQNC)-F from ATP-agarose beads, inhibiting the ATPase activity of both wild-type and mutant BtuCD-F[1].
AMP-PNP lithium hydrate (1.9-2 mM, 0-15 min) fails to inhibit the ATP-dependent scanning process of the 5' leader sequence of eukaryotic mRNA[2].
AMP-PNP lithium hydrate (1 μM-1 mM) concentration-dependently blocked the single ATP-sensitive potassium channels (KATP channels) in the skeletal muscles of mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Appearance Solid
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Molecular Weight 506.20 (free acid)
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Formula C10H17N6O12P3·xLi·yH2O
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Color White to light yellow
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP(OP(NP(O)(O)=O)(O)=O)(O)=O.[x].[Li].[y].O
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Synonyms
Adenylyl imidodiphosphate lithium hydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Mol Cell
2025 Mar 20;85(6):1176-1188.e5. PMID: 40010342
Solvent & Solubility
In Vitro:
H2O : 50 mg/mL (Need ultrasonic and warming)
Protocols
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Immunoprecipitation
Immunoprecipitation (IP) is an experimental method that uses the principle of antibody specific binding to purify and enrich target proteins.
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Two-electrode voltage clamp in Xenopus oocytes
Two-electrode voltage clamp measures whole-oocyte membrane current from Xenopus oocytes expressing exogenous ion channels, receptors, or transporters; one intracellular microelectrode senses membrane voltage, and the second injects current so the amplifier can hold the membrane at command voltages while recording the compensating current as the functional readout. The method is suited to Xenopus oocytes because their large size supports microinjection and intracellular electrode impalement, but the large membrane area can limit voltage-clamp speed and accuracy, especially for large or fast currents.
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Co-Immunoprecipitation
Co-immunoprecipitation technology can verify protein interaction based on the specific immune reaction between antibodies and antigens.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Korkhov VM, et al. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012 Oct 18;490(7420):367-72. [Content Brief]
[2]. Sakharov PA, et al. Nonhydrolyzable ATP analog 5'-adenylyl-imidodiphosphate (AMP-PNP) does not inhibit ATP-dependent scanning of leader sequence of mRNA. Biochemistry (Mosc). 2015 Jan;80(1):45-9. [Content Brief]
[3]. Ellinghaus TL, et al. Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle. Sci Adv. 2021 Dec 24;7(52):eabk2392. [Content Brief]
[4]. Hehl S, Neumcke B. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP. Eur Biophys J. 1994;23(4):231-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AMP-PNP lithium hydrate Related Classifications
HY-130777A Related Classifications
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