AMP-PNP tetralithium
Based on 1 publication(s) in Google Scholar
AMP-PNP (Adenylyl-imidodiphosphate) tetralithium is a non-hydrolyzable ATP analog. AMP-PNP tetralithium binds to ATP binding sites competely but is not hydrolyzed by enzymes, providing stable experimental conditions for studying ATP-dependent processes. AMP-PNP tetralithium can also be used to study enzyme activity, kinase regulation, DNA/RNA metabolism, ion channel function, and protein complex assembly.
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- Reinheit: 99.92%
- CAS. Nr.: 72957-42-7
- Formel: C10H13Li4N6O12P3
- Molecular Weight:529.93
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Speicherung:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) AMP-PNP tetralithium
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Biologische Aktivität
AMP-PNP tetralithium (100 nM-3.3 mM) competitively elutes BtuCD(EQNC)-F from ATP-agarose beads, inhibiting the ATPase activity of both wild-type and mutant BtuCD-F[1].
AMP-PNP tetralithium (1.9-2 mM, 0-15 min) fails to inhibit the ATP-dependent scanning process of the 5' leader sequence of eukaryotic mRNA[2].
AMP-PNP tetralithium (1 μM-1 mM) concentration-dependently blocked the single ATP-sensitive potassium channels (KATP channels) in the skeletal muscles of mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 72957-42-7
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Appearance Solid
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Molecular Weight 529.93
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Formel C10H13Li4N6O12P3
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Color White to off-white
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SMILES
O[C@H]1[C@H](N2C3=C(N=C2)C(N)=NC=N3)O[C@@H]([C@H]1O)COP(OP(NP(O[Li])(O[Li])=O)(O[Li])=O)(O[Li])=O
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Synonyms
Adenylyl-imidodiphosphate tetralithium
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Mol Cell
2025 Mar 20;85(6):1176-1188.e5. PMID: 40010342
Lösungsmittel & Löslichkeit
H2O : 26.5 mg/mL (50.01 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Korkhov VM, et al. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012 Oct 18;490(7420):367-72. [Content Brief]
[2]. Sakharov PA, et al. Nonhydrolyzable ATP analog 5'-adenylyl-imidodiphosphate (AMP-PNP tetralithium) does not inhibit ATP-dependent scanning of leader sequence of mRNA. Biochemistry (Mosc). 2015 Jan;80(1):45-9. [Content Brief]
[3]. Ellinghaus TL, et al. Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle. Sci Adv. 2021 Dec 24;7(52):eabk2392. [Content Brief]
[4]. Hehl S, Neumcke B. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP tetralithium. Eur Biophys J. 1994;23(4):231-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8870 mL | 9.4352 mL | 18.8704 mL | 47.1760 mL |
| 5 mM | 0.3774 mL | 1.8870 mL | 3.7741 mL | 9.4352 mL | |
| 10 mM | 0.1887 mL | 0.9435 mL | 1.8870 mL | 4.7176 mL | |
| 15 mM | 0.1258 mL | 0.6290 mL | 1.2580 mL | 3.1451 mL | |
| 20 mM | 0.0944 mL | 0.4718 mL | 0.9435 mL | 2.3588 mL | |
| 25 mM | 0.0755 mL | 0.3774 mL | 0.7548 mL | 1.8870 mL | |
| 30 mM | 0.0629 mL | 0.3145 mL | 0.6290 mL | 1.5725 mL | |
| 40 mM | 0.0472 mL | 0.2359 mL | 0.4718 mL | 1.1794 mL | |
| 50 mM | 0.0377 mL | 0.1887 mL | 0.3774 mL | 0.9435 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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