AMP-PNP lithium hydrate  (Synonyms: Adenylyl imidodiphosphate lithium hydrate)

AMP-PNP (Adenylyl imidodiphosphate) lithium hydrate is a non-hydrolyzable ATP analog. AMP-PNP lithium hydrate binds to ATP binding sites competely but is not hydrolyzed by enzymes, providing stable experimental conditions for studying ATP-dependent processes. AMP-PNP lithium hydrate can also be used to study enzyme activity, kinase regulation, DNA/RNA metabolism, ion channel function, and protein complex assembly^[1][2][3][4].

AMP-PNP lithium hydrate (100 nM-3.3 mM) competitively elutes BtuCD(EQNC)-F from ATP-agarose beads, inhibiting the ATPase activity of both wild-type and mutant BtuCD-F^[1].
AMP-PNP lithium hydrate (1.9-2 mM, 0-15 min) fails to inhibit the ATP-dependent scanning process of the 5' leader sequence of eukaryotic mRNA^[2].
AMP-PNP lithium hydrate (1 μM-1 mM) concentration-dependently blocked the single ATP-sensitive potassium channels (KATP channels) in the skeletal muscles of mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

506.20 (free acid)

C₁₀H₁₇N₆O₁₂P₃·xLi·yH₂O

Solid

White to light yellow

O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP(OP(NP(O)(O)=O)(O)=O)(O)=O.[x].[Li].[y].O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Korkhov VM, et al. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012 Oct 18;490(7420):367-72.  [Content Brief]

  [2]. Sakharov PA, et al. Nonhydrolyzable ATP analog 5'-adenylyl-imidodiphosphate (AMP-PNP) does not inhibit ATP-dependent scanning of leader sequence of mRNA. Biochemistry (Mosc). 2015 Jan;80(1):45-9.  [Content Brief]

  [3]. Ellinghaus TL, et al. Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle. Sci Adv. 2021 Dec 24;7(52):eabk2392.  [Content Brief]

  [4]. Hehl S, Neumcke B. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP. Eur Biophys J. 1994;23(4):231-7.  [Content Brief]