Anthracene-9-carboxylic acid
Based on 1 Customer Validation
Anthracene-9-carboxylic acid (9-Anthracenecarboxylic acid) is an anthracene derivative traditionally used to block and identify Ca2+-activated Cl- currents (CaCCs) in various cell types, like diverse smooth muscle cells, epithelial cells and salivary gland cells.
For research use only. We do not sell to patients.
- Purity: 99.21%
- CAS No.: 723-62-6
- Formula: C15H10O2
- Molecular Weight:222.24
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
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Biological Activity
Ca2+-activated Cl- currents[1]
Anthracene-9-carboxylic acid causes a voltage-dependent block of outward currents in HEK 293T cells and inhibits a larger fraction of the current as depolarization increased[1].
Anthracene-9-carboxylic acid induces a strong potentiation of tail currents measured at -100 mV after depolarizing voltages, as well as a prolongation of the deactivation kinetics in HEK 293T[1].
Anthracene-9-carboxylic acid (500 μM, rabbit pulmonary artery smooth muscle cells) produces a small inhibition of the maximum outward Cl- current at +70 mV (21±10%) but augmented the amplitude of the instantaneous inward relaxation at -80 mV by 321±34%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 723-62-6
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Appearance Solid
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Molecular Weight 222.24
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Formula C15H10O2
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Color Light yellow to yellow
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SMILES
O=C(C1=C2C=CC=CC2=CC3=CC=CC=C13)O
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Synonyms
9-Anthracenecarboxylic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (449.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (22.50 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Cherian OL, Menini A, Boccaccio A. Multiple effects of anthracene-9-carboxylic acid on the TMEM16B/anoctamin2 calcium-activated chloride channel. Biochim Biophys Acta. 2015 Apr;1848(4):1005-13. [Content Brief]
[2]. Piper AS, Greenwood IA. Anomalous effect of anthracene-9-carboxylic acid on calcium-activated chloride currents in rabbit pulmonary artery smooth muscle cells. Br J Pharmacol. 2003 Jan;138(1):31-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.4996 mL | 22.4982 mL | 44.9964 mL | 112.4910 mL |
| 5 mM | 0.8999 mL | 4.4996 mL | 8.9993 mL | 22.4982 mL | |
| 10 mM | 0.4500 mL | 2.2498 mL | 4.4996 mL | 11.2491 mL | |
| 15 mM | 0.3000 mL | 1.4999 mL | 2.9998 mL | 7.4994 mL | |
| 20 mM | 0.2250 mL | 1.1249 mL | 2.2498 mL | 5.6245 mL | |
| 25 mM | 0.1800 mL | 0.8999 mL | 1.7999 mL | 4.4996 mL | |
| 30 mM | 0.1500 mL | 0.7499 mL | 1.4999 mL | 3.7497 mL | |
| 40 mM | 0.1125 mL | 0.5625 mL | 1.1249 mL | 2.8123 mL | |
| 50 mM | 0.0900 mL | 0.4500 mL | 0.8999 mL | 2.2498 mL | |
| 60 mM | 0.0750 mL | 0.3750 mL | 0.7499 mL | 1.8748 mL | |
| 80 mM | 0.0562 mL | 0.2812 mL | 0.5625 mL | 1.4061 mL | |
| 100 mM | 0.0450 mL | 0.2250 mL | 0.4500 mL | 1.1249 mL |
Anthracene-9-carboxylic acid Related Classifications
HY-101329 Related Classifications
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