Anti-IGFBP2 Antibody (M14)

Cat. No.: HY-P991042 Purity: ≥99.0%: Data Sheet
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Anti-IGFBP2 Antibody (M14) is an human anti-IGFBP2 monoclonal inhibitory antibody, which binds IGFBP2 with high affinity and blocks its binding with IGF1. Anti-IGFBP2 Antibody (M14) inhibits human endothelial cell recruitment, thus blocks the tumor progression of human metastatic cancer.

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1 mg		In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description	Anti-IGFBP2 Antibody (M14) is an human anti-IGFBP2 monoclonal inhibitory antibody, which binds IGFBP2 with high affinity and blocks its binding with IGF1. Anti-IGFBP2 Antibody (M14) inhibits human endothelial cell recruitment, thus blocks the tumor progression of human metastatic cancer^[1].
Isotype	Human IgG1 kappa
Recommend Isotype Controls	Human IgG1 kappa, Isotype Control
Species Reactivity	Human
Gene ID	3485 [NCBI]
Accession	P18065
Application	ELISA, FACS, Functional assay
Conjugated	Unconjugated
Reconsititution	The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight	146.7 kDa
Appearance	Liquid
Color	Colorless to light yellow
SMILES	[Anti-IGFBP2 Antibody (M14)]
Shipping	Shipping with dry ice.
Formulation	Please refer to the lot-specific COA for specific buffer information.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity	Flow cytometric analysis of 1X10⁵⁰ MCF-7 cells with Anti-IGFBP2 Antibody (HY-P991042, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Purity & Documentation	Purity: ≥99.0% Select Batch: Data Sheet (254 KB) SDS (251 KB) COA (232 KB) SDS-PAGE (67 KB) SEC-HPLC (94 KB) Inhibitory Antibodies User Guide (603 KB)
References	[1]. TAVAZOIE S, et al., Treatment of angiogenesis disorders. WO2012109567A2.

Anti-IGFBP2 Antibody (M14) Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-IGFBP2 Antibody (M14)IGF-1RInsulin-like growth factor-1 receptorangiogenesisMDA-MB-231NOD-SCID mouseInhibitorinhibitorinhibit

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Flow cytometric analysis of 1X10⁵⁰ MCF-7 cells with Anti-IGFBP2 Antibody (HY-P991042, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
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