Antibacterial agent 348
Antibacterial agent 348 is an antimicrobial agent containing piperazine-isopropanolamine bifunctional groups. Antibacterial agent 348 suppresses virulence factor expression in plant pathogens. Antibacterial agent 348 compromises bacterial membrane integrity, leading to cytoplasmic leakage. Antibacterial agent 348 inhibits cellular proliferation of plant pathogens, diminishes colonization and infectivity of plant pathogens in host plants. Antibacterial agent 348 can be used for the research of rice bacterial leaf blight, pepper phytophthora blight.
For research use only. We do not sell to patients.
- Formula: C33H38FN3O4
- Molecular Weight:559.67
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
Antibacterial agent 348 (Compound Q17) potently inhibits Phytophthora capsica (p.c.) mycelial growth with an EC50 of 9.8 μg/mL[1].
Antibacterial agent 348 potently inhibits Xanthomonas oryzae pv oryzae (Xoo) growth with an EC50 of 0.9 μg/mL, achieving complete inhibition at 100 μg/mL[1].
Antibacterial agent 348 (100 μg/mL) potently inhibits Xanthomonas axonopodis pv citri (Xac) growth with an EC50 of 11.9 μg/mL, achieving 98.3% inhibition at 100 μg/mL[1].
Antibacterial agent 348 (100 μg/mL) inhibits Xanthomonas campestris pv Mangifera indica (Xcm) growth by 75.1% at 100 μg/mL[1].
Antibacterial agent 348 (100 μg/mL) inhibits Acidovorax citrulli (Ac) growth by 79.0% at 100 μg/mL[1].
Antibacterial agent 348 (100 μg/mL) potently inhibits Sclerotinia sclerotiorum (S.s) mycelial growth at 100 μg/mL[1].
Antibacterial agent 348 (100 μg/mL) inhibits Botryosphaeria dothidea (B.d) mycelial growth by 82.6-84.5% at 100 μg/mL[1].
Antibacterial agent 348 (100 μg/mL) inhibits Fusarium graminearum (F.g) mycelial growth by 89.2% at 100 μg/mL[1].
Antibacterial agent 348 (25-50 μg/mL) causes concentration-dependent morphological damage to P.c mycelium, with severe shrinkage and growth inhibition observed at 50 μg/mL[1].
Antibacterial agent 348 (50 μg/mL) disrupts the structural integrity of Xoo cells at 50 μg/mL, causing cell rupture and membrane collapse[1].
Antibacterial agent 348 (12.5-100 μg/mL) induces concentration-dependent cytoplasmic leakage in P.c mycelium, damaging cell membrane integrity[1].
Antibacterial agent 348 (6.25-50 μg/mL) inhibits P.c mycelial growth in a concentration-dependent manner, reducing mycelial biomass at concentrations as low as 6.25 μg/mL[1].
Antibacterial agent 348 (0.39-25 μg/mL) inhibits Xoo growth in a concentration-dependent manner, nearly halting growth at concentrations ≥12.5 μg/mL[1].
Antibacterial agent 348 (25-100 μg/mL) reduces Xoo extracellular polysaccharide production in a concentration-dependent manner, with near-complete suppression at 100 μg/mL[1].
Antibacterial agent 348 (50-200 μg/mL) suppresses Xoo extracellular cellulase and amylase activities in a concentration-dependent manner, with maximum inhibition at 200 μg/mL[1].
Antibacterial agent 348 (0.39-25 μg/mL) disrupts Xoo biofilm formation in a concentration-dependent manner, achieving 97.1% inhibition at 12.5 μg/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
Molecular Weight 559.67
-
Formula C33H38FN3O4
-
SMILES
O=C1C(OCCCN2CCN(CC(O)CNCC3=CC=C(F)C=C3)CC2)=C(C4=CC=C(C)C=C4)OC5=C1C=CC=C5
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
-
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antibacterial agent 348 Related Classifications
HY-183966 Related Classifications
Powered by Bioz
Keywords
- Antibacterial agent 348
- Antibacterial agent348
- Antibacterial agent-348
- Bacterial
- Fungal
- Xanthomonas oryzae pv oryzae
- Sclerotinia sclerotiorum
- Phytophthora capsica
- Xanthomonas axonopodis pv citri
- Acidovorax citrulli
- pepper phytophthora blight
- Xanthomonas campestris pv Mangifera indica
- Fusarium graminearum
- Botryosphaeria dothidea
- rice bacterial leaf blight
- Inhibitor
- inhibitor
- inhibit