CD59 Antibody (YA4692)

Cat. No.: HY-P85000: Data Sheet User Guide for Antibodies
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CD59 Antibody (YA4692) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD59.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD59 Antibody (YA4692) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD59.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human

SwissProt ID

P13987

Gene ID

966 [NCBI]

Immunogen

Purified recombinant Human CD59

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-100000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in Phosphate-buffered solution.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed:11882685, PubMed:1698710, PubMed:2475111, PubMed:2475570, PubMed:2606909, PubMed:9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed:11882685, PubMed:1698710, PubMed:36797260); The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Secreted

Isoforms & Post-Translational Modification：P13987 has 2 isomers: P13987-1: 14177 Da (predicted); P13987-2: 14529 Da (predicted).
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants;Glycated (PubMed:10805801). Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes (PubMed:10805801)

Subunit：Interacts with T-cell surface antigen CD2

Synonyms

CD59; MIC11; MIN1; MIN2; MIN3; MSK21; CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin; CD59; CD59 nanobody;

Documentation

User Guide for Antibodies (1077 KB)