CHREBP/MLXIPL Antibody (YA7086)(PBS only)

Cat. No.: HY-P87398A: Data Sheet User Guide for Antibodies
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CHREBP/MLXIPL Antibody (YA7086) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHREBP/MLXIPL.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CHREBP/MLXIPL Antibody (YA7086) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHREBP/MLXIPL.

Host

Mouse

Molecular Weight

Predicted band size: 93.1kDa;

Species Reactivity

Human

SwissProt ID

Q9NP71

Immunogen

Purified recombinant fragment of human MLXIPL (AA: 18-143) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Binds DNA as a heterodimer with MLX/TCFL4 and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation (By similarity). Regulates transcription in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and gluconeogenesis, respectively (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_Specificity: Expressed in liver, heart, kidney, cerebellum and intestinal tissues

Isoforms & Post-Translational Modification：Q9NP71 has 6 isomers: Q9NP71-1: 93073 Da (predicted); Q9NP71-2: 90924 Da (predicted); Q9NP71-3: 92842 Da (predicted); Q9NP71-4: 90694 Da (predicted); Q9NP71-5: 62026 Da (predicted); Q9NP71-6: 78360 Da (predicted).
Phosphorylation at Ser-556 by AMPK inactivates the DNA-binding activity

Subunit：Binds DNA as a heterodimer with MLX/TCFL4

Synonyms

BHLHD14, MIO, WBSCR14, MLXIPL, Carbohydrate-responsive element-binding protein, ChREBP, Class D basic helix-loop-helix protein 14, MLX interactor, MLX-interacting protein-like, WS basic-helix-loop-helix leucine zipper protein, Williams-Beuren syndrome chromosomal region 14 protein, bHLHd14, WS-bHLH

Documentation

User Guide for Antibodies (1077 KB)