DDX4 Antibody (YA1208)(PBS only)

Cat. No.: HY-P81463A: Data Sheet User Guide for Antibodies
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DDX4 Antibody (YA1208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDX4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DDX4 Antibody (YA1208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDX4.

Host

Rabbit

Molecular Weight

Predicted band size: 79 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NQI0

Gene ID

54514 [NCBI]

Immunogen

Recombinant protein of human DDX4

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity)

Subcellular Localization：Cytoplasm; Cytoplasm, perinuclear region

Expression：
Tissue_specificity:It is expressed only in the ovaries and testes. It is also expressed in migratory primordial germ cells in the gonadal crest region of both sexes.

Isoforms & Post-Translational Modification：Q9NQI0 has 4 isomers: Q9NQI0-1: 79308 Da (predicted); Q9NQI0-2: 75821 Da (predicted); Q9NQI0-3: 63211 Da (predicted); Q9NQI0-4: 77056 Da (predicted).

Subunit：Interacts with RANBP9 (PubMed:27622290). Interacts with RANBP10. Interacts with PIWIL2 and MAEL.

Research Field

Epigenetics and Nuclear Signaling

Synonyms

VASA; MGC111074; DDX4; MVH

Documentation

User Guide for Antibodies (1077 KB)