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  3. Primary Antibodies
  4. Monoclonal Antibodies Flow Cytometry Antibodies
  5. FBXO31 Antibody (YA7869)

FBXO31 Antibody (YA7869)

製品番号: HY-P88185
User Guide for Antibodies Technical Support

FBXO31 Antibody (YA7869) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to FBXO31.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

容量 価格（税別） 在庫状況
20 μL $115 見積もりとリードタイムを求めるのご確認
50 μL $185 見積もりとリードタイムを求めるのご確認
100 μL $300 見積もりとリードタイムを求めるのご確認

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

FBXO31 Antibody (YA7869) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to FBXO31.
Host

Mouse
Clonality

Monoclonal
分子量

Predicted band size: 60.5 kDa
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Immunogen

Full length human recombinant protein of human FBXO31 produced in HEK293T cell.
Application &
Dilution Ratio
Application Dilution Ratio
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-250
FC
FC: Flow Cytometry
1:100
Sensitivity Endogenous 純度 Affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG2b  
Appearance

Liquid
Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Substrate-recognition component of the SCF(FBXO31) protein ligase complex, which specifically mediates the ubiquitination of proteins amidated at their C-terminus in response to oxidative stress, leading to their degradation by the proteasome (PubMed:39880951). FBXO31 specifically recognizes and binds C-terminal peptides bearing an amide: C-terminal amidation in response to oxidative stress takes place following protein fragmentation (PubMed:39880951). The SCF(FBXO31) also plays a role in G1 arrest following DNA damage by mediating ubiquitination of phosphorylated cyclin-D1 (CCND1), promoting its degradation by the proteasome, resulting in G1 arrest (PubMed:19412162, PubMed:29279382). The SCF(FBXO31) complex is however not a major regulator of CCND1 stability during the G1/S transition (By similarity). In response to genotoxic stress, the SCF(FBXO31) complex directs ubiquitination and degradation of phosphorylated MDM2, thereby promoting p53/TP53-mediated DNA damage response (PubMed:26124108). SCF(FBXO31) complex is required for genomic integrity by catalyzing ubiquitination and degradation of cyclin-A (CCNA1 and/or CCNA2) during the G1 phase (PubMed:31413110). In response to genotoxic stress, the SCF(FBXO31) complex directs ubiquitination and degradation of phosphorylated FBXO46 and MAP2K6 (PubMed:24936062, PubMed:30171069). SCF(FBXO31) complex promotes ubiquitination and degradation of CDT1 during the G2 phase to prevent re-replication (PubMed:24828503). The SCF(FBXO31) complex also mediates ubiquitination and degradation of DUSP6, OGT and PARD6A (PubMed:23469015, PubMed:34686346, PubMed:39894887)
Subcellular Localization：Cytoplasm,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Expression：
Tissue_Specificity: Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow
Induction: By DNA damage. Increases after UV irradiation, X-ray irradiation, oxidative stress (H(2)O(2)) or addition of the chemotherapeutic DNA-damaging agents etoposide, adriamycin, cisplatin or fluorouracil
Isoforms & Post-Translational Modification：Q5XUX0 has two isomers: Q5XUX0-1: 60664 Da (predicted); Q5XUX0-2: 49039 Da (predicted).
Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization (PubMed:19412162)丨Ubiquitinated by the SCF(FBXO46) complex in absence of stress, promoting its degradation (PubMed:30171069)
Subunit：Part of a SCF (SKP1-cullin-F-box) protein ligase complex SCF(FBXO31) composed of CUL1, SKP1, RBX1 and FBXO31 (PubMed:16357137, PubMed:19412162, PubMed:31413110, PubMed:39880951)
Synonyms
FBX14; Fbx31; FBXO14; MRT45; pp2386
ドキュメンテーション
User Guide for Antibodies (1077 KB)

FBXO31 Antibody (YA7869) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
FBXO31 Antibody (YA7869)
製品番号:
HY-P88185
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