HIPK1 Antibody (YA8185)(PBS only)

Cat. No.: HY-P88501A: Data Sheet User Guide for Antibodies
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HIPK1 Antibody (YA8185) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HIPK1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HIPK1 Antibody (YA8185) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HIPK1.

Host

Mouse

Molecular Weight

Predicted band size: 87.1 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q86Z02

Immunogen

Human recombinant protein fragment corresponding to amino acids 439-697 of human HIPK1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN (PubMed:24559171)

Subcellular Localization：Nucleus,Cytoplasm,Nucleus speckle

Expression：
Tissue_Specificity: Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis. Expressed in both androgen-dependent and androgen-independent prostate cancer cells (PubMed:28289210)

Isoforms & Post-Translational Modification：Q86Z02 has four isomers: Q86Z02-1: 130843 Da (predicted); Q86Z02-2: 116731 Da (predicted); Q86Z02-3: 89481 Da (predicted); Q86Z02-4: 87302 Da (predicted).
Autophosphorylated丨Degraded by PARK7 at the protein level丨Sumoylated

Subunit：Interacts with Nkx1-2, Nkx2-5, MYB, PARK7, DAXX and p53/TP53

Synonyms

Myak; Nbak2

Documentation

User Guide for Antibodies (1077 KB)